Biocompatibility and biomechanical characteristics of loofah based scaffolds combined with hydroxyapatite, cellulose, poly-L-lactic acid with chondrocyte-like cells

Basit Öğe Kaydı
dc.contributor.authorCecen, Berivan
dc.contributor.authorKozaci, Leyla Didem
dc.contributor.authorYuksel, Mithat
dc.contributor.authorUstun, Ozcan
dc.contributor.authorErgur, Bekir Ugur
dc.contributor.authorHavitcioglu, Hasan
dc.date.accessioned2019-10-27T11:24:27Z
dc.date.available2019-10-27T11:24:27Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractThe current study reports the biocompatibility and biomechanical characteristics of loofah-based scaffolds combined with hydroxyapatite (HA), cellulose, poly-L-lactic acid (PLLA) with chondrocytes-like cells. Scanning electron microscope (SEM) micrographs of the scaffolds showed that the addition of PLLA usually resulted in an increase in cell's attachment on scaffolds. Mechanical and elemental analyzes were assessed using tensile test and Energy Dispersive X-ray spectrometry (EDS), respectively. In summary, we showed that the loofah + PLLA + HA scaffolds perform significantly better than other loofah-based scaffolds employed in terms of increasing a diversity of mechanical properties including tensile strength and Young's modulus. Based on the analysis of the differential scanning calorimetry (DSC) thermograms and EDS spectrums that give an idea about the calcium phosphate (CaP) ratios, the improvement in the mechanical properties could principally be recognized to the strong interaction formed between loofah, PLLA and HA. The viability of chondrocytes on loofah-based scaffolds was analyzed by XTT tests. However, none of the scaffolds have proved to be toxic in metabolic activity. The histological evaluation obtained by hematoxylin and eosin (H&E), Masson trichrome, toluidine blue and immunohistochemistry methods showed that cells in all scaffolds produced extracellular matrix that defined proteoglycan and type I-II collagens. The results of this study suggest that the loofah-based scaffold with desirable properties can be considered as an ideal candidate for cartilage tissue engineering applications. (C) 2016 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipDepartment of Biostatistics and Medical Informaticsen_US
dc.description.sponsorshipThe authors would like to thank Dr. Filiz OZMIHCI from IYTE-Chemistry Engineering who helped during the DSC studies of the thesis and provided opportunity. Authors also thank Associate Professor Dr. Pembe KESKINOGLU, at the Department of Biostatistics and Medical Informatics, for the support she has given statistically. I would like to thank Elvan Dogan for arranging of high resolution images.en_US
dc.identifier.doi10.1016/j.msec.2016.07.007en_US
dc.identifier.endpage446en_US
dc.identifier.issn0928-4931
dc.identifier.issn1873-0191
dc.identifier.pmid27612733en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage437en_US
dc.identifier.urihttps://doi.org/10.1016/j.msec.2016.07.007
dc.identifier.urihttps://hdl.handle.net/11454/33281
dc.identifier.volume69en_US
dc.identifier.wosWOS:000383930900052en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofMaterials Science & Engineering C-Materials For Biological Applicationsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSW-1353en_US
dc.subjectCelluloseen_US
dc.subjectLoofahen_US
dc.subjectPoly-L-lactic aciden_US
dc.subjectHydroxyapatiteen_US
dc.titleBiocompatibility and biomechanical characteristics of loofah based scaffolds combined with hydroxyapatite, cellulose, poly-L-lactic acid with chondrocyte-like cellsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu