Ionic liquid-reinforced Hydroxyapatite@nano-TiO2 as a green platform for Immuno-electrochemical sensing applications

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dc.contributor.authorGhorbanizamani, Faezeh
dc.contributor.authorMoulahoum, Hichem
dc.contributor.authorTimur, Suna
dc.date.accessioned2024-08-31T07:49:26Z
dc.date.available2024-08-31T07:49:26Z
dc.date.issued2024
dc.departmentEge Üniversitesien_US
dc.description.abstractIn contemporary society, developing dependable point-of-care (POC) biosensors for the timely detection of cancer markers is crucial. Among various sensor types, screen-printed electrode (SPE)-based sensors, particularly electrochemical ones, stand out as promising candidates for POC applications. Despite ongoing efforts to create numerous SPE-based sensors, there is a continuous pursuit to enhance their sensitivity and analytical capabilities. This study presents an advanced electrochemical sensor designed to sensitively detect the hepatocellular carcinoma (HCC) marker Alpha-fetoprotein (AFP) in saliva. The sensor employs a gold SPE modified with hydroxyapatite, TiO2 2 nanoparticles, 1-butyl-3-methylimidazolium bis(trifluoromethyl sulfonyl)imide ionic liquid (IL), and AFP monoclonal antibodies. After thorough characterization and optimization using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), the biosensor exhibited a broad detection range (0.01-400 ng/ mL), a low limit of detection (LOD) at 0.058 ng/mL, and demonstrated high selectivity, repeatability, reproducibility, and stability. Furthermore, when tested with spiked human saliva samples, the biosensor displayed excellent recovery and robustness, showcasing its potential for noninvasive and POC diagnosis of HCC. In an environmentally conscious evaluation, the biosensor's greenness was assessed using the AGREE metric, yielding a high score of 0.85. This score indicates the biosensor's alignment with the principles of green analytical chemistry, underlining its eco-friendly attributes. This innovative electrochemical sensor contributes to the ongoing efforts for efficient and reliable POC diagnostic tools and aligns with a broader commitment to developing environmentally friendly solutions.en_US
dc.description.sponsorshipRepublic of Turkiye, Ministry of Development supported the infrastructure of EGE MATAL (Ege University/Izmir) [2016K121190]; Scientific and Technological Research Institution of Turkiye (TUBITAK) [123O186]en_US
dc.description.sponsorshipThe Republic of Turkiye, Ministry of Development supported the infrastructure of EGE MATAL (Ege University/Izmir) via the 2016K121190 grant. The study was partly supported by the Scientific and Technological Research Institution of Turkiye (TUBITAK grant number 123O186) .en_US
dc.identifier.doi10.1016/j.talanta.2024.126688
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.pmid39128315en_US
dc.identifier.scopus2-s2.0-85200805300en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2024.126688
dc.identifier.urihttps://hdl.handle.net/11454/104872
dc.identifier.volume280en_US
dc.identifier.wosWOS:001294527400001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofTalantaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240831_Uen_US
dc.subjectIonic Liquiden_US
dc.subjectHydroxyapatiteen_US
dc.subjectTio2en_US
dc.subjectScreen-Printed Electrodeen_US
dc.subjectBiosensoren_US
dc.subjectElectrochemistryen_US
dc.titleIonic liquid-reinforced Hydroxyapatite@nano-TiO2 as a green platform for Immuno-electrochemical sensing applicationsen_US
dc.typeArticleen_US

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