The comparison of microscopy and real time polymerase chain reaction methods for the diagnosis of pneumocystis jirovecii pneumonia: Evaluation of clinical parameters [Pneumocystis jirovecii pnömonisi tanısında mikroskopi ve gerçek zamanlı polimeraz zincir reaksiyonu yöntemlerinin karşılaştırılması: Klinik bulgular ile yorumlanması]
Küçük Resim Yok
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Ankara University
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Introduction: Pneumocystis jirovecii pneumonia (PCP) causes serious infections, especially in patients with immunosuppressive diseases. In this study, it was aimed to evaluate the results of samples obtained from PCP suspected patients using two different methods together with clinical data. Materials and Methods: Microscopy and real time polymerase chain reaction (real time PCR) methods were performed with bronchoalveolar lavage (BAL) samples sended to Ege University Medical Faculty Direct Parasitology Diagnostic Laboratory between March 2009 and June 2010. Demographic characteristics, clinical and laboratory data were also recorded retrospectively. The data were evaluated using the SPSS 16.0 program. Results: A total of 42 BAL samples collected from patients (24 males, mean age: 31.49 ± 26.14) were included. There were totally 16 P. jirovecii positives either one of the tests. Sixteen and three samples were detected positive by real time PCR and microscopy, respectively. Trimethoprim-sulfamethoxazole was prescribed in 11 PCP diagnosed cases and 6 of them died. Conclusion: Today, despite the growing opportunities in diagnosis and treatment, PCP pneumonia is associated with high mortality. Careful examination of clinical data and immune status of the patients are important. Multidisciplinary approach is required for early PCP diagnosis. © 2017, Ankara University. All rights reserved.
Açıklama
Anahtar Kelimeler
Pneumocystis jirovecii, Pneumonia, Real time PCR
Kaynak
Tuberkuloz ve Toraks
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
65
Sayı
3
