Electrochemical Investigation of Curcumin-DNA Interaction by Using Hydroxyapatite Nanoparticles-Ionic Liquids Based Composite Electrodes
Küçük Resim Yok
Tarih
2021
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Mdpi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Hydroxyapatite nanoparticles (HaP) and ionic liquid (IL) modified pencil graphite electrodes (PGEs) are newly developed in this assay. Electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and cyclic voltammetry (CV) were applied to examine the microscopic and electrochemical characterization of HaP and IL-modified biosensors. The interaction of curcumin with nucleic acids and polymerase chain reaction (PCR) samples was investigated by measuring the changes at the oxidation signals of both curcumin and guanine by differential pulse voltammetry (DPV) technique. The optimization of curcumin concentration, DNA concentration, and the interaction time was performed. The interaction of curcumin with PCR samples was also investigated by gel electrophoresis.
Açıklama
Anahtar Kelimeler
hydroxyapatite nanoparticles, ionic liquid, bionanomaterials, nucleic acid interactions, curcumin, electrochemical biosensors, Graphite-Electrodes, Biosensor, Sensor, Immobilization, Quantitation, Immunosensor, Sequence, Binding, Trends, Urine
Kaynak
Materials
WoS Q Değeri
Q1
Scopus Q Değeri
Q2
Cilt
14
Sayı
15
