Design of radiolabeled gelatinase inhibitor peptide (Tc-99m-CLP) and evaluation in rats

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dc.contributor.authorAltiparmak, Burcu
dc.contributor.authorLambrecht, Fatma Yurt
dc.contributor.authorCitak, Asli
dc.date.accessioned2019-10-27T22:14:14Z
dc.date.available2019-10-27T22:14:14Z
dc.date.issued2014
dc.departmentEge Üniversitesien_US
dc.description.abstractIn malignant tissues, MMP-9 (gelatinase B, 92 kDa type IV collagenase) and MMP-2 (gelatinase A, 72 kDa type IV collagenase) are the most prevalent matrix metalloproteinases related to the tumor aggressiveness and metastatic potential. Since elevated levels of gelatinases are associated with poor prognosis in cancer patients, these enzymes are potential targets for tumor imaging to possibly predict metastases. In the present study, a cyclic decapeptide, CLP (Cys-Leu-Pro-Gly-His-Trp-Gly-Phe-Pro-Ser-Cys), was selected as a basic peptide because of its selective inhibitory activity toward gelatinases. The peptide was labelled with Tc-99m with a radiolabelling efficiency of 94.6 +/- 4.1%. After determining the appropriate conditions for radiolabelling, a biodistribution study of radiolabelled peptide in Albino Wistar rats was done. According to biodistribution data, Tc-99m-CLP showed high uptake in the lung, liver, uterus and spleen. The amount of normal tissue MMPs enzymes is known to be lower than a tumor tissue. In this connection, our findings show that matrix metalloproteinases inhibitory peptide which is CLP is labeled with Tc-99m with high yield and radiolabeled peptide might be might be utilized for the imaging of gelatinase activity due to overexpression of MMP-2 and MMP-9 in tumor tissue. (C) 2014 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipDepartment of Scientific Projects at Ege University, Izmir, TurkeyEge University [NBE 001]; Scientific and Technological Research Council of Turkey, Scientific ProjectTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [111S253]en_US
dc.description.sponsorshipThe authors gratefully acknowledge the financial support received from Department of Scientific Projects at Ege University, Izmir, Turkey (2011 NBE 001) and the Scientific and Technological Research Council of Turkey, Scientific Project (111S253).en_US
dc.identifier.doi10.1016/j.apradiso.2014.02.012en_US
dc.identifier.endpage133en_US
dc.identifier.issn0969-8043
dc.identifier.pmid24631744en_US
dc.identifier.startpage130en_US
dc.identifier.urihttps://doi.org/10.1016/j.apradiso.2014.02.012
dc.identifier.urihttps://hdl.handle.net/11454/49966
dc.identifier.volume89en_US
dc.identifier.wosWOS:000338401200020en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofApplied Radiation and Isotopesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGelatinaseen_US
dc.subjectRadiolabeled peptideen_US
dc.subjectMatrix metalloproteinasesen_US
dc.subjectHWGF-containing peptideen_US
dc.titleDesign of radiolabeled gelatinase inhibitor peptide (Tc-99m-CLP) and evaluation in ratsen_US
dc.typeArticleen_US

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