Antifungal and Antibiofilm Activities of Selective Serotonin Reuptake Inhibitors Alone and in Combination with Fluconazole

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dc.contributor.authorTekintas, Yamac
dc.contributor.authorTemel, Aybala
dc.contributor.authorAtes, Aysegul
dc.contributor.authorErac, Bayri
dc.contributor.authorMetin, Dilek YeIm
dc.contributor.authorPolat, Suleyha Hilmioglu
dc.contributor.authorLimoncu, Mine Hosgor
dc.date.accessioned2021-05-03T20:36:43Z
dc.date.available2021-05-03T20:36:43Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractObjectives: Candida spp. are clinically important pathogens that cause difficulties for treatment by biofilm formation. Considering antifungal resistance rates and the limitations in the discovery of new antifungals, the antifungal and antibiofilm effects of various drugs used for different therapeutic purposes are becoming more important. The goal of our study was to determine the antifungal and antibiofilm effects of the selective serotonin reuptake inhibitors (SSRIs), namely sertraline (SRT), paroxetine (PRX), and fluoxetine (FLX) alone and in combination with fluconazole (FLC) against Candida spp. Materials and Methods: Twenty Candida spp. strains isolated from clinical samples from Ege University Hospital were identified by the Dalmau method and matrix-assisted laser desorption ionization time of flight mass spectrometry. The minimum inhibitory concentrations (MICs) of the SSRIs and FLC were detected by broth microdilution method. Synergistic interactions between the SSRIs and FLC were investigated by checkerboard assay. The antibiofilm effects of the SSRIs were determined by spectrophotometric microplate method. Results: Among the isolates, five different Candida spp. (C. albicans, C. glabrata, C. krusei, C. tropicalis, and C.parapsilosis) were identified. The MICs of the SSRIs ranged between 16-512 mu g/mL. While SRT showed the highest antifungal effect, the antibiofilm efficacy of FLX was higher than that of the other agents. Moreover, FLX and PRX showed a synergistic effect with FLC in 13 and 19 isolates, respectively. Four isolates were strong biofilm producers while nine isolates were moderate biofilm producers. C. parapsilosis strains showed higher biofilm production than the other species. At MIC/2 concentration, FLX and SRT alone inhibited mature biofilms in six and five isolates, respectively, while PRX caused increases biofilm formation in seven isolates. Conclusion: This study revealed that MIC/2 concentrations of SSRIs could have antifungal and antibiofilm effects. SRT and FLX alone or in combination with antifungals may possibly have therapeutic potential for combating fungal infections.en_US
dc.identifier.doi10.4274/tjps.galenos.2019.65481en_US
dc.identifier.endpage672en_US
dc.identifier.issn1304-530X
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85098631841en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage667en_US
dc.identifier.urihttps://doi.org/10.4274/tjps.galenos.2019.65481
dc.identifier.urihttps://hdl.handle.net/11454/70163
dc.identifier.volume17en_US
dc.identifier.wosWOS:000605355000014en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTurkish Pharmacists Assocen_US
dc.relation.ispartofTurkish Journal of Pharmaceutical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCandida spp.en_US
dc.subjectfluconazoleen_US
dc.subjectEUCASTen_US
dc.subjectsynergistic effecten_US
dc.subjectantibiofilmen_US
dc.titleAntifungal and Antibiofilm Activities of Selective Serotonin Reuptake Inhibitors Alone and in Combination with Fluconazoleen_US
dc.typeArticleen_US

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