Effects of cyanocobalamin and its combination with morphine on neuropathic rats and the relationship between these effects and thrombospondin-4 expression

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dc.authoridSengul, Gulgun/0000-0002-5826-7379
dc.authoridDuzenli, Neslihan/0000-0001-6764-4423
dc.authorscopusid57201364949
dc.authorscopusid6701495848
dc.authorscopusid37032923000
dc.authorscopusid57428097100
dc.authorscopusid7004825492
dc.authorwosidSengul, Gulgun/AAE-8601-2020
dc.contributor.authorDuzenli, Neslihan
dc.contributor.authorUlker, Sibel
dc.contributor.authorSengul, Gulgun
dc.contributor.authorKayhan, Buse
dc.contributor.authorOnal, Aytul
dc.date.accessioned2023-01-12T20:15:21Z
dc.date.available2023-01-12T20:15:21Z
dc.date.issued2022
dc.departmentN/A/Departmenten_US
dc.description.abstractBackground: Thrombospondin-4 (TSP4) upregulates in the spinal cord following peripheral nerve injury and contributes to the development of neuropathic pain (NP). We investigated the effects of cyanocobalamin alone or in combination with morphine on pain and the relationship between these effects and spinal TSP4 expression in neuropathic rats. Methods: NP was induced by chronic constriction injury (CCI) of the sciatic nerve. Cyanocobalamin (5 and 10 mg/kg/day) was administered 15 days before CCI and then for 4 and 14 postoperative days. Morphine (2.5 and 5 mg/kg/day) was administered only post-CCI. Combination treatment included cyanocobalamin and morphine, 10 and 5 mg/kg/day, respectively. All drugs were administered intraperitoneally. Nociceptive thresholds were detected by esthesiometer, analgesia meter, and plantar test, and TSP4 expression was assessed by western blotting and fluorescence immunohistochemistry. Results: CCI decreased nociceptive thresholds in all tests and induced TSP4 expression on the 4th postoperative day. The decrease in nociceptive thresholds persisted except for the plantar test, and the increased TSP4 expression reversed on the 14th postoperative day. Cyanocobalamin and low-dose morphine alone did not produce any antinociceptive effects. High-dose morphine improved the decreased nociceptive thresholds in the esthesiometer when administered alone but combined with cyanocobalamin in all tests. Cyanocobalamin and morphine significantly induced TSP4 expression when administered alone in both doses for 4 or 14 days. However, this increase was less when the two drugs are combined. Conclusions: The combination of cyanocobalamin and morphine is more effective in antinociception and partially decreased the induced TSP4 expression compared to the use of either drug alone.en_US
dc.description.sponsorshipScientific Research Fund of Ege University [TGA-2018-20111]en_US
dc.description.sponsorshipThis study was supported by the Scientific Research Fund of Ege University (Project No: TGA-2018-20111) .en_US
dc.identifier.doi10.3344/kjp.2022.35.1.66
dc.identifier.endpage77en_US
dc.identifier.issn2005-9159
dc.identifier.issn2093-0569
dc.identifier.issue1en_US
dc.identifier.pmid34966013en_US
dc.identifier.scopus2-s2.0-85123550240en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage66en_US
dc.identifier.urihttps://doi.org/10.3344/kjp.2022.35.1.66
dc.identifier.urihttps://hdl.handle.net/11454/78439
dc.identifier.volume35en_US
dc.identifier.wosWOS:000739836200008en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherKorean Pain Socen_US
dc.relation.ispartofKorean Journal of Painen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAnalgesicsen_US
dc.subjectOpioiden_US
dc.subjectCobamidesen_US
dc.subjectMorphineen_US
dc.subjectNeuralgiaen_US
dc.subjectPainen_US
dc.subjectPeripheral Nerve Injuriesen_US
dc.subjectRatsen_US
dc.subjectSciatic Nerveen_US
dc.subjectSpinal Corden_US
dc.subjectThrombospondinsen_US
dc.subjectVitamin B 12en_US
dc.subjectSciatic-Nerve Crushen_US
dc.subjectAcidic Protein Gfapen_US
dc.subjectPain Sensation Likeen_US
dc.subjectSpinal Sensitizationen_US
dc.subjectInjuryen_US
dc.subjectContributesen_US
dc.subjectVitamin-B12en_US
dc.subjectAstrocytesen_US
dc.subjectMononeuropathyen_US
dc.subjectRegenerationen_US
dc.titleEffects of cyanocobalamin and its combination with morphine on neuropathic rats and the relationship between these effects and thrombospondin-4 expressionen_US
dc.typeArticleen_US

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