Optimization of beta-galactosidase production using Kluyveromyces lactis NRRL Y-8279 by response surface methodology

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dc.contributor.authorDagbagli, Seval
dc.contributor.authorGoksungur, Yekta
dc.date.accessioned2019-10-27T20:23:23Z
dc.date.available2019-10-27T20:23:23Z
dc.date.issued2008
dc.departmentEge Üniversitesien_US
dc.description.abstractThis paper investigates the production and optimization of beta-galactosidase enzyme using synthetic medium by Kluyveromyces lactis NRRL Y-8279 in shake flask cultures. Among the different cell disintegration methods used, the highest specific activity was obtained when the cells were permeabilized using isoamyl alcohol. Response surface methodology was used to investigate the effects of four fermentation parameters (agitation speed, pH, initial substrate concentration and incubation time) on beta-galactosidase enzyme production. Results of the statistical analysis showed that the fit of the model was good in all cases. Maximum specific enzyme activity of 4218.4 U g(-1) was obtained at the optimum levels of process variables (pH 7.35, agitation speed 179.2 rpm, initial sugar concentration 24.9 g l(-1) and incubation time 50.9 hrs). The response surface methodology was found to be useful in optimizing and determining the interactions among process variables in beta-galactosidase enzyme production.en_US
dc.description.sponsorshipState Planning Organization (Turkey)Turkiye Cumhuriyeti Kalkinma Bakanligi [DPT. 2005K120570]; Scientific and Technical Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TOVAG 104 O 270]en_US
dc.description.sponsorshipThis research was supported by the State Planning Organization (Turkey) through Project no: DPT. 2005K120570 and Scientific and Technical Research Council of Turkey (TUBITAK) through Project no: TOVAG 104 O 270.en_US
dc.identifier.doi10.2225/vol11-issue4-fulltext-12
dc.identifier.issn0717-3458
dc.identifier.issn0717-3458en_US
dc.identifier.issue4en_US
dc.identifier.urihttps://doi.org/10.2225/vol11-issue4-fulltext-12
dc.identifier.urihttps://hdl.handle.net/11454/42012
dc.identifier.volume11en_US
dc.identifier.wosWOS:000262927300012en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherUniv Catolica De Valparaisoen_US
dc.relation.ispartofElectronic Journal of Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbeta-galactosidaseen_US
dc.subjectKluyveromyces lactisen_US
dc.subjectresponse surface methodologyen_US
dc.subjectshake flask cultureen_US
dc.subjectsynthetic mediumen_US
dc.titleOptimization of beta-galactosidase production using Kluyveromyces lactis NRRL Y-8279 by response surface methodologyen_US
dc.typeArticleen_US

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