Optimization of beta-galactosidase production using Kluyveromyces lactis NRRL Y-8279 by response surface methodology
Küçük Resim Yok
Tarih
2008
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Univ Catolica De Valparaiso
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
This paper investigates the production and optimization of beta-galactosidase enzyme using synthetic medium by Kluyveromyces lactis NRRL Y-8279 in shake flask cultures. Among the different cell disintegration methods used, the highest specific activity was obtained when the cells were permeabilized using isoamyl alcohol. Response surface methodology was used to investigate the effects of four fermentation parameters (agitation speed, pH, initial substrate concentration and incubation time) on beta-galactosidase enzyme production. Results of the statistical analysis showed that the fit of the model was good in all cases. Maximum specific enzyme activity of 4218.4 U g(-1) was obtained at the optimum levels of process variables (pH 7.35, agitation speed 179.2 rpm, initial sugar concentration 24.9 g l(-1) and incubation time 50.9 hrs). The response surface methodology was found to be useful in optimizing and determining the interactions among process variables in beta-galactosidase enzyme production.
Açıklama
Anahtar Kelimeler
beta-galactosidase, Kluyveromyces lactis, response surface methodology, shake flask culture, synthetic medium
Kaynak
Electronic Journal of Biotechnology
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
11
Sayı
4
