Cobalt Phthalocyanine-Ionic Liquid Composite Modified Electrodes for the Voltammetric Detection of DNA Hybridization Related to Hepatitis B Virus

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dc.authoridErdem, Arzum/0000-0002-4375-8386
dc.authorscopusid57204189653
dc.authorscopusid7004459748
dc.authorwosidErdem, Arzum/X-1925-2019
dc.contributor.authorYarali, Ece
dc.contributor.authorErdem, Arzum
dc.date.accessioned2023-01-12T20:15:41Z
dc.date.available2023-01-12T20:15:41Z
dc.date.issued2021
dc.departmentN/A/Departmenten_US
dc.description.abstractIn this study, cobalt phthalocyanine (CoPc) and ionic liquid (IL) modified pencil graphite electrodes (PGEs) were designed and implemented to detect sequence-selective DNA hybridization related to the Hepatitis B virus (HBV). The surface characterization of CoPc-IL-PGEs was investigated by scanning electron microscopy (SEM), and the electrochemical behavior of electrodes were studied by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. The voltammetric detection of hybridization was investigated by evaluating the guanine oxidation signal, measured by differential pulse voltammetry (DPV) technique. The implementation of our biosensor to serum samples was also examined using fetal bovine serum (FBS). The detection limit was established as 0.19 mu g/mL in phosphate buffer solution (PBS) (pH 7.40) and 2.48 mu g/mL in FBS medium. The selectivity of our assay regarding HBV DNA hybridization in FBS medium was tested in the presence of other DNA sequences. With this aim, the hybridization of DNA probe with non-complementary (NC) or mismatched DNA sequence (MM), or in the presence of mixture samples containing DNA target NC (1:1) or DNA target MM (1:1), was studied based on the changes in guanine signal.en_US
dc.description.sponsorshipTurkish Academy of Sciences (TUBA)en_US
dc.description.sponsorshipA.E. would like to express her gratitude to the Turkish Academy of Sciences (TUBA) as a Principal member for its partial support.en_US
dc.identifier.doi10.3390/mi12070753
dc.identifier.issn2072-666X
dc.identifier.issue7en_US
dc.identifier.pmid34206863en_US
dc.identifier.scopus2-s2.0-85109570891en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.3390/mi12070753
dc.identifier.urihttps://hdl.handle.net/11454/78545
dc.identifier.volume12en_US
dc.identifier.wosWOS:000676293500001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofMicromachinesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectcobalt phthalocyanineen_US
dc.subjectionic liquiden_US
dc.subjectvoltammetric biosensoren_US
dc.subjectHepatitis B virusen_US
dc.subjectpencil graphite electrodeen_US
dc.subjectdifferential pulse voltammetryen_US
dc.subjectSingle-Use Sensoren_US
dc.subjectCarbon Nanotubesen_US
dc.subjectElectrochemical Detectionen_US
dc.subjectImpedance Spectroscopyen_US
dc.subjectGraphite-Electrodesen_US
dc.subjectBiosensoren_US
dc.subjectFilmen_US
dc.subjectNanoparticlesen_US
dc.subjectSurfaceen_US
dc.titleCobalt Phthalocyanine-Ionic Liquid Composite Modified Electrodes for the Voltammetric Detection of DNA Hybridization Related to Hepatitis B Virusen_US
dc.typeArticleen_US

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