The effect of autologous bone marrow stromal cells differentiated on scaffolds for canine tibial bone reconstruction

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dc.contributor.authorOzdal-Kurt F.
dc.contributor.authorTu?lu I.
dc.contributor.authorVatansever H.
dc.contributor.authorTong S.
dc.contributor.authorDelilo?lu-Gurhan S.
dc.date.accessioned2019-10-26T21:26:09Z
dc.date.available2019-10-26T21:26:09Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractBone marrow contains mesenchymal stem cells that form many tissues. Various scaffolds are available for bone reconstruction by tissue engineering. Osteoblastic differentiated bone marrow stromal cells (BMSC) promote osteogenesis on scaffolds and stimulate bone regeneration. We investigated the use of cultured autologous BMSC on different scaffolds for healing defects in tibias of adult male canines. BMSC were isolated from canine humerus bone marrow, differentiated into osteoblasts in culture and loaded onto porous ceramic scaffolds including hydroxyapatite 1, hydroxyapatite gel and calcium phosphate. Osteoblast differentiation was verified by osteonectine and osteocalcine immunocytochemistry. The scaffolds with stromal cells were implanted in the tibial defect. Scaffolds without stromal cells were used as controls. Sections from the defects were processed for histological, ultrastructural, immunohistochemical and histomorphometric analyses to analyze the healing of the defects. BMSC were spread, allowed to proliferate and differentiate to osteoblasts as shown by alizarin red histochemistry, and osteocalcine and osteonectine immunostaining. Scanning electron microscopy showed that BMSC on the scaffolds were more active and adhesive to the calcium phosphate scaffold compared to the others. Macroscopic bone formation was observed in all groups, but scaffolds with stromal cells produced significantly better results. Bone healing occurred earlier and faster with stromal cells on the calcium phosphate scaffold and produced more callus compared to other scaffolds. Tissue healing and osteoblastic marker expression also were better with stromal cells on the scaffolds. Increased trabecula formation, cell density and decreased fibrosis were observed in the calcium phosphate scaffold with stromal cells. Autologous cultured stromal cells on the scaffolds were useful for healing of canine tibial bone defects. The calcium phosphate scaffold was the best for both cell differentiation in vitro and bone regeneration in vivo. It may be possible to improve healing of bone defects in humans using stem cells from bone marrow. © 2015 The Biological Stain Commission.en_US
dc.description.sponsorshipFlorida Education Funden_US
dc.description.sponsorshipThis project was partially supported by Celal Bayar University Scientific Research Projects Commission (FEF. 2003/036) and TUBITAK VHAG-2118. --en_US
dc.identifier.doi10.3109/10520295.2014.983547en_US
dc.identifier.endpage528en_US
dc.identifier.issn1052-0295
dc.identifier.issue7en_US
dc.identifier.pmid25994048en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage516en_US
dc.identifier.urihttps://doi.org/10.3109/10520295.2014.983547
dc.identifier.urihttps://hdl.handle.net/11454/17321
dc.identifier.volume90en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor and Francis Ltden_US
dc.relation.ispartofBiotechnic and Histochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBone marrowen_US
dc.subjecthealingen_US
dc.subjectosteoblasten_US
dc.subjectstromal cellsen_US
dc.subjecttissue engineeringen_US
dc.titleThe effect of autologous bone marrow stromal cells differentiated on scaffolds for canine tibial bone reconstructionen_US
dc.typeArticleen_US

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