Potential neuroprotective effect of gamma-glutamylcysteine ethyl ester on rat brain against kainic acid-induced excitotoxicity

Basit Öğe Kaydı
dc.contributor.authorYalcin, Ayfer
dc.contributor.authorArmagan, Guliz
dc.contributor.authorTurunc, Ezgi
dc.contributor.authorKonyalioglu, Sibel
dc.contributor.authorKanit, Lutfiye
dc.date.accessioned2019-10-27T21:17:54Z
dc.date.available2019-10-27T21:17:54Z
dc.date.issued2010
dc.departmentEge Üniversitesien_US
dc.description.abstractThe aim of this study was to investigate the effect of gamma-Glutamylcysteine Ethyl Ester (GCEE) on the levels of GSH, caspase-3 activity, DNA damage and the expressions of Bcl-2, Bax and p53 mRNAs in rat hippocampus after status epilepticus (SE) induced by systemic kainic acid (KA). The male rats were divided into four groups as controls, KA (10 mg/kg), GCEE (10 mg/kg) and KA+GCEE. Glutathione (GSH) levels and caspase-3 activity were determined spectrophotometrically and colourimetrically, respectively. DNA damage and Bcl-2, Bax and p53 mRNA expressions were quantified by comet assay and reverse transcription followed by RT-PCR, respectively. KA treatment significantly depleted GSH levels, induced DNA damage, caspase-3 activity and the expressions of p53 and Bax mRNA. GCEE treatment protected GSH levels, decreased DNA damage and the levels of p53 and Bax/Bcl-2 mRNA against KA injection. These results indicate that GCEE treatment at the dose of 10 mg/kg is capable to protect the depleted levels of GSH and shows an anti-apoptotic activity due to the decreased levels of apoptotic biomarkers in the rat hippocampus after SE induced by KA.en_US
dc.description.sponsorshipTechnological and Scientific Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [SBAG-K69-104S280]en_US
dc.description.sponsorshipThis study was supported by the Technological and Scientific Council of Turkey (TUBITAK) (SBAG-K69-104S280 to A.Y). G. A and E. T were also supported by doctoral grants from TUBITAK. The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.en_US
dc.identifier.doi10.3109/10715761003645964en_US
dc.identifier.endpage521en_US
dc.identifier.issn1071-5762
dc.identifier.issn1029-2470
dc.identifier.issue5en_US
dc.identifier.pmid20214503en_US
dc.identifier.startpage513en_US
dc.identifier.urihttps://doi.org/10.3109/10715761003645964
dc.identifier.urihttps://hdl.handle.net/11454/43889
dc.identifier.volume44en_US
dc.identifier.wosWOS:000276815300004en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofFree Radical Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titlePotential neuroprotective effect of gamma-glutamylcysteine ethyl ester on rat brain against kainic acid-induced excitotoxicityen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu