The effect of clomiphene citrate on osteoporosis in ovariectomized rats

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dc.contributor.authorUyar Y.
dc.contributor.authorKoltan S.O.
dc.contributor.authorPögün Ş.
dc.contributor.authorVatansever S.
dc.contributor.authorÇaglar H.
dc.date.accessioned2019-10-26T23:56:59Z
dc.date.available2019-10-26T23:56:59Z
dc.date.issued2008
dc.departmentEge Üniversitesien_US
dc.description.abstractObjective: The aim of this study was to investigate whether clomiphene citrate (CC) administration could be a new therapeutic agent in case of contraindication of estrogen therapy for hormone-dependent osteoporosis and to show the changes in bone structure by histomorphometric analysis in ovariectomized rats administered CC. Study design: This study was carried out in the Experimental Surgery Laboratory of the Brain Research Centre of the Medical Faculty of Ege University. Four-month-old Sprague-Dawley rats were used for the experiment. The study was carried out on six groups of animals each consisted of eight rats. Four groups of rats were ovariectomized and 2 groups of rats were used as control group. For 6 weeks every day, rats were injected physiological saline solution (1 ml/kg), clomiphene citrate (1 or 10 mg/1 ml/kg, Organon), 17ß-estradiol (50 µg/1 ml/kg, within susame oil, Sigma) or susame oil (1 ml/kg, Sigma). Drug administrations were carried out according to the weekly weight measurements. Group 1(PSS), n = 8, non-ovariectomized, were injected with physiological saline solution. Group 2(CC-1), n = 7, non-ovariectomized, were injected with CC (1 mg/1 ml/kg). Group 3(OVX + CC-1), n = 7, ovariectomized, were injected with CC (1 mg/1 ml/kg). Group 4(OVX + CC-10), n = 6, ovariectomized, were injected with CC (10 mg/1 ml/kg). Group 5(OVX + E), n = 8, ovariectomized, were injected with 17ß-estradiol (50 µg/1 ml/kg). Group 6(OVX), n = 8, ovariectomized, were injected with susame oil (1 ml/kg) Bone-specific serum alkaline phosphatase (ALP) levels were measured and statistical analyses were made by Kruskal Wallis test. Left femur bone histomorphometric studies were done. The uteri were dissected out to measure their weight and ANOVA was used to show the intergroup differences. Results: The level of ALP in group 3 was significantly higher than the other five groups. Bone histomorphometric examination showed that total bone volume in group 3, 4, and 5 was higher than group 6, and group 4 had the highest level of bone volume compared to the rest of the groups. Uterus weights in group 1 were significantly higher than group 3 and 6 (P = 0.02, P = 0.01) and uterus weights in group 5 were significantly higher than group 3 and 4 (P = 0.00, P = 0.01) Conclusions: In ovariectomized rats, treatment with CC is seen as effective as estrogen treatment in preventing osteoporosis, without causing uterin hyperstimulation. Nevertheless, further investigations on more rats are needed to assess whether it is an alternative treatment method to estrogen. © 2007 Springer-Verlag.en_US
dc.identifier.doi10.1007/s00404-007-0534-yen_US
dc.identifier.endpage114en_US
dc.identifier.issn0932-0067
dc.identifier.issue2en_US
dc.identifier.pmid18193254en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage107en_US
dc.identifier.urihttps://doi.org/10.1007/s00404-007-0534-y
dc.identifier.urihttps://hdl.handle.net/11454/20919
dc.identifier.volume278en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofArchives of Gynecology and Obstetricsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectClomiphene citrateen_US
dc.subjectEstrogenen_US
dc.subjectOvariectomized raten_US
dc.subjectPostmenopausal osteoporosisen_US
dc.titleThe effect of clomiphene citrate on osteoporosis in ovariectomized ratsen_US
dc.typeArticleen_US

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