Development of amino functionalized carbon coated magnetic nanoparticles and their application to electrochemical detection of hybridization of nucleic acids

Basit Öğe Kaydı
dc.contributor.authorAltay, Cansu
dc.contributor.authorSenay, R. Hilal
dc.contributor.authorEksin, Ece
dc.contributor.authorCongur, Gulsah
dc.contributor.authorErdem, Arzum
dc.contributor.authorAkgol, Sinan
dc.date.accessioned2019-10-27T11:10:27Z
dc.date.available2019-10-27T11:10:27Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractIn our study, the development of amino functionalized carbon coated magnetic nanoparticles (NH2-CC-MNPS) and their usage for electrochemical detection of hybridization of nucleic acids have been aimed. Firstly, NH2CC-MNPS were prepared by coating of pristine Fe3O4 nanoparticles with two layers via caramelization and silanization processes respectively. After the morphological characterization with scanning electron microscopy (SEM) it was seen that NH2-CC-MNPs was spherical shaped and in 28 nm sized. Investigation of chemical composition with the help of scanning electron microscopy/energy dispersive X-ray spectroscopy (SEM/EDX) and fourier transform infrared spectroscopy (FTIR) was showed incorporation of carbon and APTES to the structure of NH2-CC-MNPs. Magnetic property of NH2-CC-MNPs after two layered coatings was demonstrated with electron spin resonance (ESR) technique and gfactor was calculated as 2.6. In the second part of this study, optimization studies have carried out onto the surface of NH2-CC-MNPS prepared in saltless phosphate-tween 20 buffer (PBTw) for the analysis of DNA hybridization. The thiol linked DNA probe sequence representing to the Hepatitis B virus (HBV) concentration, target DNA sequence concentration, the most productive hybridization time and the selection of the nanoparticle surfaces have been researched. The electrochemical detection of DNA hybridization was investigated using PGE in combination with differential pulse voltammetry (DPV) technique by measuring the guanine oxidation signal. The detection limit was calculated in the linear target DNA concentration range of 5-25 rho/mL and it was found to be 1.15 mu g/mL (20 pmol in 110 mu L solution). It has been intended to be more reproducible, more sensitive and faster results with developed biosensor technology.en_US
dc.description.sponsorshipEge University Scientific Research Project CoordinationEge University [13/FBE/010]; Turkish Academy of Sciences (TUBA)Turkish Academy of Sciencesen_US
dc.description.sponsorshipA.E acknowledges the financial support from Ege University Scientific Research Project Coordination (Project no.13/FBE/010), and she also would like to express her gratitude to the Turkish Academy of Sciences (TUBA) as the Principal member for its partial support.en_US
dc.identifier.doi10.1016/j.talanta.2016.11.012en_US
dc.identifier.endpage182en_US
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.pmid28107914en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage175en_US
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2016.11.012
dc.identifier.urihttps://hdl.handle.net/11454/32395
dc.identifier.volume164en_US
dc.identifier.wosWOS:000393244400026en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofTalantaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAmino functionalized magnetic nanoparticlesen_US
dc.subjectDisposable graphite electrodeen_US
dc.subjectElectrochemical biosensoren_US
dc.subjectDifferential pulse voltammetryen_US
dc.subjectHBV DNAen_US
dc.titleDevelopment of amino functionalized carbon coated magnetic nanoparticles and their application to electrochemical detection of hybridization of nucleic acidsen_US
dc.typeArticleen_US

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