Combination of resveratrol and BIBR1532 inhibits proliferation of colon cancer cells by repressing expression of LncRNAs

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dc.authoridGunduz, Cumhur/0000-0002-6593-3237
dc.authoridAvci, Cigir Biray/0000-0001-8251-4520
dc.authoridCaglar, Hasan Onur/0000-0002-3637-4755
dc.authorscopusid57204243449
dc.authorscopusid56798628500
dc.authorscopusid55776206000
dc.authorscopusid57203689522
dc.authorscopusid6603599022
dc.authorscopusid57212299393
dc.authorwosidGunduz, Cumhur/GOP-0629-2022
dc.authorwosidBiray Avcı, Cigir/GWV-1665-2022
dc.contributor.authorCesmeli, Selin
dc.contributor.authorBagca, Bakiye Goker
dc.contributor.authorCaglar, Hasan Onur
dc.contributor.authorOzates, Neslihan Pinar
dc.contributor.authorGunduz, Cumhur
dc.contributor.authorAvci, Cigir Biray
dc.date.accessioned2023-01-12T19:51:20Z
dc.date.available2023-01-12T19:51:20Z
dc.date.issued2022
dc.departmentN/A/Departmenten_US
dc.description.abstractColorectal cancer (CRC) is the third most common cancer worldwide. The development of tumor drug resistance is observed in the treatment of CRC. Combinations of anticancer agents are attracting considerable interest in order to overcome drug resistance in CRC. This study aims to investigate the effect of resveratrol and BIBR1532, either alone or in combination, on the cell viability as well as on expression of long non-coding RNAs (LncRNAs) for HT-29 colon adenocarcinoma cells. The cytotoxic effects of resveratrol and BIBR1532 on HT-29 cells were determined using WST-1 test. Flow cytometry was used to determine apoptotic cell death after treatments. Real-Time PCR was used to identify expression of LncRNAs after treatments. LncExpDB and GEPIA2 were used to evaluate expression profiles of LncRNAs, whose expression levels were decreased in HT-29 cells after treatments, in normal tissues and colon adenocarcinoma tumors. IC50 concentrations of BIBR1532 and resveratrol were found to be 50.81 mu M at 48 h and 86.23 mu M at 72 h, respectively. Combination index value was 1.07617. BIBR1532, resveratrol, or their combination reduced the cell viability of HT-29 cells. CCAT1, CRNDE, HOTAIR, PCAT1, PVT1, SNHG16 were down-regulated after treatments. In silico analysis revealed that LncRNAs whose expression levels were decreased after treatments were associated with CRC. Resveratrol, BIBR1532, or their combination may have anti-proliferative effect on colorectal cancer cells through repressing expression of LncRNAs that are involved in progression of CRC.en_US
dc.description.sponsorshipEge University Research Fund [TYL-2019-20515]en_US
dc.description.sponsorshipThis study was sponsored by Ege University Research Fund, Project No. TYL-2019-20515 (to Cigir Biray Avci).en_US
dc.identifier.doi10.1007/s12032-021-01611-w
dc.identifier.issn1357-0560
dc.identifier.issn1559-131X
dc.identifier.issue1en_US
dc.identifier.pmid34779924en_US
dc.identifier.scopus2-s2.0-85119042181en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1007/s12032-021-01611-w
dc.identifier.urihttps://hdl.handle.net/11454/76257
dc.identifier.volume39en_US
dc.identifier.wosWOS:000718816400001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherHumana Press Incen_US
dc.relation.ispartofMedical Oncologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBIBR1532en_US
dc.subjectColorectal canceren_US
dc.subjectResveratrolen_US
dc.subjectLong non-coding RNAen_US
dc.subjectHT29 cellsen_US
dc.subjectTelomerase Activityen_US
dc.subjectColorectal-Canceren_US
dc.subjectGrowth Inhibitionen_US
dc.subjectDown-Regulationen_US
dc.subjectPoor-Prognosisen_US
dc.subjectCycle Arresten_US
dc.subjectApoptosisen_US
dc.subjectCarcinomaen_US
dc.subjectFluorouracilen_US
dc.subjectOxaliplatinen_US
dc.titleCombination of resveratrol and BIBR1532 inhibits proliferation of colon cancer cells by repressing expression of LncRNAsen_US
dc.typeArticleen_US

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