Biofunctional quantum dots as fluorescence probe for cell-specific targeting

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dc.contributor.authorAg, Didem
dc.contributor.authorBongartz, Rebecca
dc.contributor.authorDogan, Leyla Eral
dc.contributor.authorSeleci, Muharrem
dc.contributor.authorWalter, Johanna-G.
dc.contributor.authorDemirkol, Dilek Odaci
dc.contributor.authorStahl, Frank
dc.contributor.authorOzcelik, Serdar
dc.contributor.authorTimur, Suna
dc.contributor.authorScheper, Thomas
dc.date.accessioned2019-10-27T22:05:23Z
dc.date.available2019-10-27T22:05:23Z
dc.date.issued2014
dc.departmentEge Üniversitesien_US
dc.description.abstractWe describe here the synthesis, characterization, bioconjugation, and application of water-soluble thioglycolic acid TGA-capped CdTe/CdS quantum dots (TGA-QDs) for targeted cellular imaging. Antihuman epidermal growth factor receptor 2 (HER2) antibodies were conjugated to TGA-QDs to target HER2-overexpressing cancer cells. TGA-QDs and TGA-QDs/anti-HER2 bioconjugates were characterized by fluorescence and UV-Vis spectroscopy, X-ray diffraction (XRD), hydrodynamic sizing, electron microscopy, and gel electrophoresis. TGA-QDs and TGA-QDs/anti-HER2 were incubated with cells to examine cytotoxicity, targeting efficiency, and cellular localization. The cytotoxicity of particles was measured using an MIT assay and the no observable adverse effect concentration (NOAEC), 50% inhibitory concentration (IC50), and total lethal concentration (TLC) were calculated. To evaluate localization and targeting efficiency of TGA-QDs with or without antibodies, fluorescence microscopy and flow cytometry were performed. Our results indicate that antibody-conjugated TGA-QDs are well-suited for targeted cellular imaging studies. (C) 2013 The Authors. Published by Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [109T573]; Federal Ministry of Education and Research (BMBF)Federal Ministry of Education & Research (BMBF) [01DL12013]; Ege UniversityEge University [2012/FEN/0071]en_US
dc.description.sponsorshipThis work is supported by Scientific and Technological Research Council of Turkey (TUBITAK, project number 109T573) and Federal Ministry of Education and Research (BMBF, project number 01DL12013). Also it was partially funded by Ege University Scientific Research Project (2012/FEN/0071). We are grateful to Michael Meyer (Gottfried Wilhelm Leibniz University of Hannover, Institute for Technical Chemistry) for his knowledge and time spent carrying out flow cytometry experiments. In addition our thanks go to F. B. Barlas (Ege University, Faculty of Science, Biochemistry department) for taking fluorescence microscope images.en_US
dc.identifier.doi10.1016/j.colsurfb.2013.09.033en_US
dc.identifier.endpage103en_US
dc.identifier.issn0927-7765
dc.identifier.issn1873-4367
dc.identifier.pmid24176888en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage96en_US
dc.identifier.urihttps://doi.org/10.1016/j.colsurfb.2013.09.033
dc.identifier.urihttps://hdl.handle.net/11454/48401
dc.identifier.volume114en_US
dc.identifier.wosWOS:000331596100013en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofColloids and Surfaces B-Biointerfacesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectQuantum dotsen_US
dc.subjectAnti-HER2en_US
dc.subjectBioconjugationen_US
dc.subjectCell specific targetingen_US
dc.subjectImagingen_US
dc.titleBiofunctional quantum dots as fluorescence probe for cell-specific targetingen_US
dc.typeArticleen_US

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