Differences and Similarities between Colorectal Cancer Cells and Colorectal Cancer Stem Cells: Molecular Insights and Implications

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dc.authoridAcikgoz, Eda/0000-0002-6772-3081
dc.authoridAsker Abdikan, Cemile Sinem/0000-0002-7515-6240
dc.authoridYesin, Taha Kadir/0000-0002-3789-3290
dc.authoridErisik, Derya/0000-0002-8199-6051
dc.authorscopusid58544152500
dc.authorscopusid56301503200
dc.authorscopusid56364984200
dc.authorscopusid58544660500
dc.authorscopusid58544324900
dc.authorscopusid8633854300
dc.contributor.authorErisik, Derya
dc.contributor.authorÖzdil, Berrin
dc.contributor.authorAçıkgöz, Eda
dc.contributor.authorAsker Abdikan, Cemile Sinem
dc.contributor.authorYasin, Taha Kadir
dc.contributor.authorAktuğ, Huseyin
dc.date.accessioned2024-08-25T18:51:28Z
dc.date.available2024-08-25T18:51:28Z
dc.date.issued2023
dc.departmentEge Üniversitesien_US
dc.description.abstractMalignant tumors are formed by diverse groups of cancercells.Cancer stem cells (CSCs) are a subpopulation of heterogeneous cellsidentified in tumors that have the ability to self-renew and differentiate.Colorectal cancer (CRC), the third most frequent malignant tumor,is progressively being supported by evidence suggesting that CSCsare crucial in cancer development. We aim to identify molecular differencesbetween CRC cells and CRC CSCs, as well as the effects of those differenceson cell behavior in terms of migration, EMT, pluripotency, morphology,cell cycle/control, and epigenetic characteristics. The HT-29 cellline (human colorectal adenocarcinoma) and HT-29 CSCs (HT-29 CD133(+)/CD44(+) cells) were cultured for 72 h. The levelsof E-cadherin, KLF4, p53, p21, p16, cyclin D2, HDAC9, and P300 proteinexpression were determined using immunohistochemistry staining. Themigration of cells was assessed by employing the scratch assay technique.Additionally, the scanning electron microscopy method was used toexamine the morphological features of the cells, and their peripheral/centralelemental ratios were compared with the help of EDS. Furthermore,a Muse cell cycle kit was utilized to determine the cell cycle analysis.The HT-29 CSC group exhibited high levels of expression for E-cadherin,p53, p21, p16, cyclin D2, HDAC9, and P300, whereas KLF4 was foundto be high in the HT-29. The two groups did not exhibit any statisticallysignificant differences in the percentages of cell cycle phases. Theidentification of specific CSC characteristics will allow for earliercancer detection and the development of more effective precision oncologyoptions.en_US
dc.identifier.doi10.1021/acsomega.3c02681
dc.identifier.endpage30157en_US
dc.identifier.issn2470-1343
dc.identifier.issue33en_US
dc.identifier.pmid37636966en_US
dc.identifier.scopus2-s2.0-85168461397en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage30145en_US
dc.identifier.urihttps://doi.org/10.1021/acsomega.3c02681
dc.identifier.urihttps://hdl.handle.net/11454/102601
dc.identifier.volume8en_US
dc.identifier.wosWOS:001045008600001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmer Chemical Socen_US
dc.relation.ispartofAcs Omegaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240825_Gen_US
dc.subjectEpithelial-Mesenchymal Transitionen_US
dc.subjectColon-Canceren_US
dc.subjectPancreatic-Canceren_US
dc.subjectHigh Expressionen_US
dc.subjectInitiating Cellsen_US
dc.subjectDown-Regulationen_US
dc.subjectCycle Arresten_US
dc.subjectGrowthen_US
dc.subjectP300en_US
dc.subjectMarkersen_US
dc.titleDifferences and Similarities between Colorectal Cancer Cells and Colorectal Cancer Stem Cells: Molecular Insights and Implicationsen_US
dc.typeArticleen_US

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