Immobilization of inulinase on concanavalin A-attached super macroporous cryogel for production of high-fructose syrup

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dc.contributor.authorAltunbaş C.
dc.contributor.authorUygun M.
dc.contributor.authorUygun D.A.
dc.contributor.authorAkgöl S.
dc.contributor.authorDenizli A.
dc.date.accessioned2019-10-27T08:23:28Z
dc.date.available2019-10-27T08:23:28Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractIn this study, concanavalin A (Con A)-attached poly(ethylene glycol dimethacrylate) [poly(EGDMA)] cryogels were used for immobilization of Aspergillus niger inulinase. For this purposes, the monolithic cryogel column was prepared by radical cryocopolymerization of EGDMA as a monomer and N,N'-methylene bisacrylamide as a crosslinker. Then, Con A was attached by covalent binding onto amino-activated poly(EGDMA) cryogel via glutaraldehyde activation. Characterization of cryogels was performed by FTIR, EDX, and SEM studies. Poly(EGDMA) cryogels were highly porous and pore size was found to be approximately 50-100 µm. Con A-attached poly(EGDMA) cryogels was used in the adsorption of inulinase from aqueous solutions. Adsorption of inulinase on the Con A-attached poly(EGDMA) cryogel was performed in continuous system and the effects of pH, inulinase concentration, and flow rate on adsorption were investigated. The maximum amount of inulinase adsorption was calculated to be 27.85 mg/g cryogel at 1.0 mg/mL inulinase concentration and in acetate buffer at pH 4.0. Immobilized inulinase was effectively used in continuous preparation of high-fructose syrup. Inulin was converted to fructose in a continuous system and released fructose concentration was found to be 0.23 mg/mL at the end of 5 min of hydrolysis. High-fructose content of the syrup was demonstrated by thin layer chromatography. © 2013 Springer Science+Business Media New York.en_US
dc.identifier.doi10.1007/s12010-013-0322-zen_US
dc.identifier.endpage1921en_US
dc.identifier.issn0273-2289
dc.identifier.issue8en_US
dc.identifier.pmid23780342en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1909en_US
dc.identifier.urihttps://doi.org/10.1007/s12010-013-0322-z
dc.identifier.urihttps://hdl.handle.net/11454/26432
dc.identifier.volume170en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofApplied Biochemistry and Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectConcanavalin Aen_US
dc.subjectCryogelen_US
dc.subjectFructoseen_US
dc.subjectInulinaseen_US
dc.subjectLectin affinity chromatographyen_US
dc.titleImmobilization of inulinase on concanavalin A-attached super macroporous cryogel for production of high-fructose syrupen_US
dc.typeArticleen_US

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