In vitro evaluation of Spirulina platensis extract incorporated skin cream with its wound healing and antioxidant activities

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dc.contributor.authorGunes, Seda
dc.contributor.authorTamburaci, Sedef
dc.contributor.authorDalay, Meltem Conk
dc.contributor.authorGurhan, Ismet Deliloglu
dc.date.accessioned2019-10-27T11:08:01Z
dc.date.available2019-10-27T11:08:01Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractContext: Algae have gained importance in cosmeceutical product development due to their beneficial effects on skin health and therapeutical value with bioactive compounds. Spirulina platensis Parachas (Phormidiaceae) is renowned as a potential source of high-value chemicals and recently used in skincare products. Objective: This study develops and evaluates skin creams incorporated with bioactive S. platensis extract. Materials and methods:Spirulina platensis was cultivated, the aqueous crude extract was prepared and in vitro cytotoxicity of S. platensis extract in the range of 0.001-1% concentrations for 1, 3 and 7d on HS2 keratinocyte cells was determined. Crude extracts were incorporated in skin cream formulation at 0.01% (w/w) concentration and in vitro wound healing and genotoxicity studies were performed. Immunohistochemical staining was performed to determine the collagen activity. Results: 0.1% S. platensis extract exhibited higher proliferation activity compared with the control group with 198% of cell viability after 3 d. Skin cream including 1.125% S. platensis crude extract showed enhanced wound healing effect on HS2 keratinocyte cell line and the highest HS2 cell viability % was obtained with this concentration. The micronucleus (MN) assay results indicated that S. platensis extract incorporated creams had no genotoxic effect on human peripheral blood cells. Immunohistochemical analysis showed that collagen 1 immunoreactivity was improved by increased extract concentration and it was strongly positive in cells treated with 1.125% extract incorporated skin cream. Conclusions: The cell viability, wound healing activity and genotoxicity results showed that S. platensis incorporated skin cream could be of potential value in cosmeceutical and biomedical applications.en_US
dc.description.sponsorshipMinistry of Science, Industry and Technology [000189.STZ.2007-2]en_US
dc.description.sponsorshipThis project was supported by The Ministry of Science, Industry and Technology [Project number: 000189.STZ.2007-2].en_US
dc.identifier.doi10.1080/13880209.2017.1331249en_US
dc.identifier.endpage1832en_US
dc.identifier.issn1388-0209
dc.identifier.issn1744-5116
dc.identifier.issue1en_US
dc.identifier.pmid28552036en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1824en_US
dc.identifier.urihttps://doi.org/10.1080/13880209.2017.1331249
dc.identifier.urihttps://hdl.handle.net/11454/32113
dc.identifier.volume55en_US
dc.identifier.wosWOS:000402314200001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofPharmaceutical Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCyanobacteriaen_US
dc.subjectcrude extracten_US
dc.subjectphycocyaninen_US
dc.subjectcytotoxicityen_US
dc.subjectgenotoxicityen_US
dc.subjectwound scratch assayen_US
dc.titleIn vitro evaluation of Spirulina platensis extract incorporated skin cream with its wound healing and antioxidant activitiesen_US
dc.typeArticleen_US

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