AML ön tanılı olgularda inv(16) CBFBETA- MYH11 inversiyonunun real time RT - PCR ile 5 yıllık sonuçlarının değerlendirilmesi
Küçük Resim Yok
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmada, Ege Üniversitesi Tıp Fakültesi Tıbbi Biyoloji Anabilim Dalı’na 2009-2013 yılları arasında akut myeloid lösemi (AML) ön tanısı ile başvuran 402 olgunun (322 yetişkin, 80 çocuk) kan veya kemik iliği örneklerinin inv16 kantitasyon analizlerinin RT-PCR yöntemi ile değerlendiril mesi amaçlandı. Gereç ve Yöntem: Hastalardan alınan kan ve kemik iliği örneklerinden total RNA/mRNA izolasyonunu takiben cDNA’ları elde edil erek revers -transkriptaz -polimeraz zincir reaksiyonu (RT-PCR) yöntemiyle inv16 kanti tasyonu LightCycler2 cihazında gerçekleştiril di . Bulgular: Dört yüz iki olgu , inv(16) CBFBETA-MYH11 inversiyonu açısından değerlendiril di . Yapılan analiz sonucunda 12 (% 4) erişkin ve 7 (% 9) çocuk olmak üzere toplam 19 olguda inv16 pozitifliği saptan dı . Sonuç: I nv16 kantitatif analizi, AML hastalarının klinik değerlendirilmesinde, hastalığın seyri ve remisyon sağlanmasına yönelik tedavi protokollerinin uygulanmasında etkili bir yöntem olduğu nu desteklemektedir. AML hasta grubunu içeren daha geniş kapsamlı çalışmalar yapılarak elde edilen sonuçlar desteklenmelidir.
Aim: In this study, it was aimed to evaluate the analysis of inv16 blood and bone marrow samples quantitive ly by reve rse transcriptase polymerase chain reaction (RT-PCR ) method of 402 patients (322 adult, 80 children) prediagnosed as acute myeloid leukemia (AML), who were admitted to Ege University Faculty of Medicine, Department of Medical Biology between years 2009 -201 3. Materials and Methods: cDNA’s were obtained following the total RNA/mRNA isolation which were taken from patients’ blood and bone marrow samples and quantitaion of inv16 were performed by reverse transcriptase RT-PCR method at LightCycler2. Results: Four hundred and two cases were evaluated in terms of inv(16) CBFBETA -MYH11 inversion. As a result of the analysis, a total of 19 patient were determined inv16 positivity includes 12 adult (% 4) and 7 children (9%).Conclusion: Inv16 of quantitative analysis is supported to be an e ffective method for clinical evaluation of patients with AML, regarding remission of disease and implimentation of the treatment protocols toward achieving remission. More studies with AML patients are required for confirmation of the results.
Aim: In this study, it was aimed to evaluate the analysis of inv16 blood and bone marrow samples quantitive ly by reve rse transcriptase polymerase chain reaction (RT-PCR ) method of 402 patients (322 adult, 80 children) prediagnosed as acute myeloid leukemia (AML), who were admitted to Ege University Faculty of Medicine, Department of Medical Biology between years 2009 -201 3. Materials and Methods: cDNA’s were obtained following the total RNA/mRNA isolation which were taken from patients’ blood and bone marrow samples and quantitaion of inv16 were performed by reverse transcriptase RT-PCR method at LightCycler2. Results: Four hundred and two cases were evaluated in terms of inv(16) CBFBETA -MYH11 inversion. As a result of the analysis, a total of 19 patient were determined inv16 positivity includes 12 adult (% 4) and 7 children (9%).Conclusion: Inv16 of quantitative analysis is supported to be an e ffective method for clinical evaluation of patients with AML, regarding remission of disease and implimentation of the treatment protocols toward achieving remission. More studies with AML patients are required for confirmation of the results.
Açıklama
Anahtar Kelimeler
Cerrahi
Kaynak
Ege Tıp Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
54
Sayı
1
