Discarded CHO cells as a valuable source of bioactive peptides for sustainable biotechnological applications

Basit Öğe Kaydı
dc.authoridTOK, Kerem/0000-0002-7244-3949
dc.contributor.authorYilmaz, Sude
dc.contributor.authorMoulahoum, Hichem
dc.contributor.authorTok, Kerem
dc.contributor.authorZihnioglu, Figen
dc.date.accessioned2024-08-31T07:47:23Z
dc.date.available2024-08-31T07:47:23Z
dc.date.issued2024
dc.departmentEge Üniversitesien_US
dc.description.abstractRepurposing discarded cells stands as a groundbreaking paradigm shift in sustainable biotechnology, with profound implications across diverse industrial sectors. Our study proposes a transformative concept by harnessing histone proteins from discarded CHO cells to produce bioactive peptides. We systematically isolated and hydrolyzed histones using Trypsin and Neutrase enzymes, optimizing reaction conditions. Ultrafiltration yielded distinct peptide fractions (<3 kDa and 3-10 kDa), which we analyzed for DPP-IV inhibition, antioxidant potential, and other activities. Furthermore, LC-Q-TOF-MS analysis and in silico tools unveiled the structural composition of bioactive peptides within these fractions. Three peptide sequences with high bioactivity potential were identified: KLPFQR, VNRFLR, and LSSCAPVFL. Our findings demonstrated exceptional DPP-IV inhibition, potent antioxidant effects, and effective anti-lipid peroxidation activities, surpassing reference compounds. Hemolytic activity assessment indicated promising biocompatibility, enhancing therapeutic application prospects. Pioneering the strategic repurposing of discarded cells, this research addresses cost-efficiency in cell-based studies and promotes sustainable use of biological resources across sectors. This novel approach offers an efficient, eco-friendly method for bioactive molecule procurement and resource management, revolutionizing cell culture studies and biotechnological applications.en_US
dc.identifier.doi10.1016/j.ijbiomac.2024.132869
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.pmid38838895en_US
dc.identifier.scopus2-s2.0-85195094482en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2024.132869
dc.identifier.urihttps://hdl.handle.net/11454/104410
dc.identifier.volume272en_US
dc.identifier.wosWOS:001252067400001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofInternational Journal of Biological Macromoleculesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmz20240831_Uen_US
dc.subjectBiotechnological Wasteen_US
dc.subjectWaste Cellsen_US
dc.subjectHistoneen_US
dc.subjectBiological Activitiesen_US
dc.subjectBiomedicineen_US
dc.titleDiscarded CHO cells as a valuable source of bioactive peptides for sustainable biotechnological applicationsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu