Production of pullulan from beet molasses and synthetic medium by Aureobasidium pullulans
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Tarih
2004
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info:eu-repo/semantics/openAccess
Özet
Bu çalışmada pancar melası ve sentetik ortamdan Aureobasidium pullulans P56 ile pullulan üretimi araştırılmıştır. Polisakkarit üretimini artırmak için melasa uygulanan ön işlemlerden sülfürik asit + aktif kömür ile daha yüksek polisakkarit, polisakkarit verimi ve şeker tüketim değerleri elde edilmiştir. Sentetik ortam ile 21.4 g $l^{-1}$ polisakkarit ve 16.7 g $l^{-1}$ pullulan, melas ile ise 35.0 g $l^{-1}$ polisakkarit ve 16.9 g $l^{-1}$ pullulan üretimi, 50 g $l^{-1}$ başlangıç şeker konsantrasyonunda (pH 7.5) elde edilmiştir. Sentetik ortamdan elde edilen toplam polisakkaritin % 85.3'ünün pullulan olduğu belirlenirken, bu oran melas içeren ortamda % 48.3'tür. Bu sonuç sentetik ortamdan yüksek saflıkta pullulan elde edilirken melastan elde edilen polisakkaritin yaklaşık yarısının pullulan dışı diğer polisakkaritlerden oluştuğunu göstermektedir. Sentetik ortam ile gerçekleştirilen denemelerde, yüksek başlangıç şeker konsantrasyonlarının pullulan veriminde azalmaya neden olduğu belirlenmiştir. Karıştırmalı tank tipi biyoreaktörde yapılan denemede, 20.4 g $l^{-1}$ polisakkarit ve çalkalamalı kültüre göre çok daha düşük bir değer olan 6.6g $l^{-1}$ pullulan konsantrasyonu elde edilmiştir.
The production of pullulan from molasses and synthetic medium by Aureobasidium pullulans P56 in batch culture was investigated. Among the pretreatments of molasses used to increase polysaccharide production, sulfuric acid + activated carbon treatment gave better results with regard to polysaccharide concentration, polysaccharide yield and sugar utilization. A maximum polysaccharide concentration of 21.4 g $l^{-1}$ and a pullulan concentration of 16.7 g $l^{-1}$ were obtained in synthetic medium whereas 35.0 g $l^{-1}$ of polysaccharide and 16.9 g $l^{-1}$ of pullulan were obtained in molasses medium containing 50 g $l^{-1}$ of initial sugar at pH 7.5. The highest pullulan contents of the crude polysaccharide were 85.3 and 48.396 for synthetic medium and molasses medium, respectively and this showed that A. pullulans produced a mixture of pullulan and other polysaccharides in molasses medium. The experiments performed with synthetic medium showed that higher initial sugar concentrations led to a significant decrease in pullulan yield. A polysaccharide concentration of 20.4 g $l^{-1}$ and a pullulan concentration of 6.6 g $l^{-1}$ were obtained in a stirred tank bioreactor with synthetic medium. These concentrations were lower than those in shake flask culture.
The production of pullulan from molasses and synthetic medium by Aureobasidium pullulans P56 in batch culture was investigated. Among the pretreatments of molasses used to increase polysaccharide production, sulfuric acid + activated carbon treatment gave better results with regard to polysaccharide concentration, polysaccharide yield and sugar utilization. A maximum polysaccharide concentration of 21.4 g $l^{-1}$ and a pullulan concentration of 16.7 g $l^{-1}$ were obtained in synthetic medium whereas 35.0 g $l^{-1}$ of polysaccharide and 16.9 g $l^{-1}$ of pullulan were obtained in molasses medium containing 50 g $l^{-1}$ of initial sugar at pH 7.5. The highest pullulan contents of the crude polysaccharide were 85.3 and 48.396 for synthetic medium and molasses medium, respectively and this showed that A. pullulans produced a mixture of pullulan and other polysaccharides in molasses medium. The experiments performed with synthetic medium showed that higher initial sugar concentrations led to a significant decrease in pullulan yield. A polysaccharide concentration of 20.4 g $l^{-1}$ and a pullulan concentration of 6.6 g $l^{-1}$ were obtained in a stirred tank bioreactor with synthetic medium. These concentrations were lower than those in shake flask culture.
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Kaynak
Turkish Journal of Biology
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Cilt
28
Sayı
1
