Indiscriminate SARS-CoV-2 multivariant detection using magnetic nanoparticle-based electrochemical immunosensing

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dc.authoridTimur, Suna/0000-0002-1981-7577
dc.authoridŞanlı, Serdar/0000-0002-9048-287X
dc.authoridMOULAHOUM, Hichem/0000-0003-3934-6415
dc.authoridharmanci, duygu/0000-0001-9133-8362
dc.authoridDurmus, Ceren/0000-0002-8648-0505
dc.authoridBALABAN HANOGLU, Simge/0000-0002-4540-1882
dc.authorscopusid57210729367
dc.authorscopusid57289928300
dc.authorscopusid55935858800
dc.authorscopusid57188963997
dc.authorscopusid57220178117
dc.authorscopusid57199103659
dc.authorscopusid57201739373
dc.authorwosidTimur, Suna/CAG-0914-2022
dc.authorwosidŞanlı, Serdar/AAG-5393-2019
dc.authorwosidEvran, Serap/AFU-1537-2022
dc.authorwosidŞANLI, SERDAR/HJI-5858-2023
dc.authorwosidTok, Kerem/AAG-6529-2021
dc.authorwosidMOULAHOUM, Hichem/DIC-3335-2022
dc.contributor.authorDurmus, Ceren
dc.contributor.authorHarmanci, Duygu
dc.contributor.authorMoulahoum, Hichem
dc.contributor.authorTok, Kerem
dc.contributor.authorGhorbanizamani, Faezeh
dc.contributor.authorSanli, Serdar
dc.contributor.authorZihnioglu, Figen
dc.date.accessioned2023-01-12T19:58:24Z
dc.date.available2023-01-12T19:58:24Z
dc.date.issued2022
dc.departmentN/A/Departmenten_US
dc.description.abstractThe increasing mutation frequency of the SARS-CoV-2 virus and the emergence of successive variants have made correct diagnosis hard to perform. Developing efficient and accurate methods to diagnose infected patients is crucial to effectively mitigate the pandemic. Here, we developed an electrochemical immunosensor based on SARS-CoV-2 antibody cocktail-conjugated magnetic nanoparticles for the sensitive and accurate detection of the SARS-CoV-2 virus and its variants in nasopharyngeal swabs. The application of the antibody cocktail was compared with commercially available anti-SARS-CoV-2 S1 (anti-S1) and anti-S2 monoclonal antibodies. After optimization and calibration, the limit of detection (LOD) determination demonstrated a LOD = 0.53-0.75 ng/ mL for the antibody cocktail-based sensor compared with 0.93 ng/mL and 0.99 ng/mL for the platforms using anti-S1 and anti-S2, respectively. The platforms were tested with human nasopharyngeal swab samples pre diagnosed with RT-PCR (10 negatives and 40 positive samples). The positive samples include the original, alpha, beta, and delta variants (n = 10, for each). The polyclonal antibody cocktail performed better than commercial anti-S1 and anti-S2 antibodies for all samples reaching 100% overall sensitivity, specificity, and accuracy. It also showed a wide range of variants detection compared to monoclonal antibody-based platforms. The present work proposes a versatile electrochemical biosensor for the indiscriminate detection of the different variants of SARS-CoV-2 using a polyclonal antibody cocktail. Such diagnostic tools allowing the detection of variants can be of great efficiency and economic value in the fight against the ever-changing SARS-CoV-2 virus.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [120R021]; Republic of Turkey, Ministry of Development [2016K121190]en_US
dc.description.sponsorshipThis study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK, Project Grant No: 120R021) and Republic of Turkey, Ministry of Development (Project Grant No: 2016K121190) . The authors are thankful to the EGE-MATAL for providing its facilities for all experimental stages.en_US
dc.identifier.doi10.1016/j.talanta.2022.123356
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.pmid35248943en_US
dc.identifier.scopus2-s2.0-85125655131en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2022.123356
dc.identifier.urihttps://hdl.handle.net/11454/76884
dc.identifier.volume243en_US
dc.identifier.wosWOS:000788739300007en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofTalantaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSARS-CoV-2en_US
dc.subjectElectrochemical biosensoren_US
dc.subjectAntigen testen_US
dc.subjectMagnetic nanoparticlesen_US
dc.subjectAntibody cocktailen_US
dc.subjectNasopharyngeal swab specimensen_US
dc.subjectAntibody Cocktailen_US
dc.subjectBiosensorsen_US
dc.subjectPlatformen_US
dc.titleIndiscriminate SARS-CoV-2 multivariant detection using magnetic nanoparticle-based electrochemical immunosensingen_US
dc.typeArticleen_US

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