Assessment of genetic markers and glioblastoma stem-like cells in activation of dendritic cells

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dc.contributor.authorYurtsever, Aysel
dc.contributor.authorHaydaroglu, Ayfer
dc.contributor.authorAvci, Cigir Biray
dc.contributor.authorGunduz, Cumhur
dc.contributor.authorOktar, Nezih
dc.contributor.authorDalbasti, Tayfun
dc.contributor.authorCaglar, Hasan Onur
dc.contributor.authorAttar, Rukset
dc.contributor.authorKitapcioglu, Gul
dc.date.accessioned2019-10-27T22:08:19Z
dc.date.available2019-10-27T22:08:19Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractGlioblastoma (GBM) is the most common and aggressive intraparenchymal primary brain tumor in adults. The principal reasons for the poor outcomes of GBM are the high rates of recurrence and resistance to chemotherapy. The aim of this study was to determine the role of tailored cellular therapy for GBM with a poor prognosis and compare the activity of dendritic cells (DCs) that have encountered GBM cells. Detecting the correlations between methylation and expression of MGMT and PTEN genes and GBM cancer stem cells (CSCs) markers after co-cultures with a mononuclear cell cocktail are also aims for this study. Allogenic umbilical cord blood (UCB)-derived DCs were labeled with the CD11a and CD123 for immature DCs, and CD80 and CD11c for mature DCs. CD34, CD45, and CD56 cells were isolated from allogenic UCB for using in DCs maturation. GBM CSCs were detected with CD133/1 and CD111 antibodies after co-culture studies. DC activation was carried out via GBM cells including CD133 and CD111 cells and a mononuclear cells cocktail including CD34, CD45, and CD56 natural killer cells. Real-time PCR was performed to detect the expression and promoter methylation status of PTEN and MGMT genes. The expression of CSCs markers was found in all GBM cases, and a statistically significant correlation was found among them after co-culture studies. The most pronounced affinity of DCs to GBM cells was observed at dilutions between 1/4 and 1/256 in co-cultures. There was a statistically significant correlation between cellularity and granularity ratios for CD123 and CD11c. PTEN and MGMT gene expression and methylation values were evaluated with respect to CSCs expression and no statistical significance was found. Activation of DCs might associate with CSCs and the mononuclear cells cocktail including CD34, CD45, and CD56 cells which were obtained from allogenic UCB.en_US
dc.description.sponsorshipONKIM Stem Cell Technologiesen_US
dc.description.sponsorshipThis study was supported by ONKIM Stem Cell Technologies. We acknowledge Pinar Huner and Cem Kulen from ONKIM Stem Cell Technologies for their contributions in the study.en_US
dc.identifier.doi10.1007/s13577-013-0065-8en_US
dc.identifier.endpage113en_US
dc.identifier.issn1749-0774
dc.identifier.issue3en_US
dc.identifier.pmid23737374en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage105en_US
dc.identifier.urihttps://doi.org/10.1007/s13577-013-0065-8
dc.identifier.urihttps://hdl.handle.net/11454/49163
dc.identifier.volume26en_US
dc.identifier.wosWOS:000328540400003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer Japan Kken_US
dc.relation.ispartofHuman Cellen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGlioblastomaen_US
dc.subjectCancer stem-like cellsen_US
dc.subjectDendritic cellsen_US
dc.subjectPTENen_US
dc.subjectMGMTen_US
dc.titleAssessment of genetic markers and glioblastoma stem-like cells in activation of dendritic cellsen_US
dc.typeArticleen_US

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