Bioethanol production from raffinate phase of supercritical CO2 extracted Stevia rebaudiana leaves
Küçük Resim Yok
Tarih
2012
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The extracts of Stevia rebaudiana are marketed as dietary supplements and utilized as natural sweetening agent in food products. Subsequent to extraction on industrial scale, large quantities of solid wastes are produced. The aim of this study was to investigate the bioconversion efficiency of supercritical CO2 extracted S. rebaudiana residues. Therefore, leaves were extracted with supercritical CO2 and ethanol mixture in order to obtain glycosides, then the raffinate phase was hydrolyzed by both dilute acid and various concentrations of cellulase and ß-glucosidase cocktail. The maximum yield of reducing sugars reached 25.67g/L under the optimal conditions of enzyme pretreatment, whereas 32.00g/L was reached by consecutive enzymatic and acid hydrolyses. Bioethanol yield (20g/L, 2.0% inoculum, 2days) based on the sugar consumed was 45.55% corresponding to a productivity of 0.19kg/m3h which demonstrates challenges to be utilized as a potential feedstock for the production of bioethanol. © 2012 Elsevier Ltd.
Açıklama
Anahtar Kelimeler
Bioethanol, Biofuel, Cellulase, Stevia, Supercritical fluid extraction
Kaynak
Bioresource Technology
WoS Q Değeri
Scopus Q Değeri
Q1
Cilt
120