Structural changes in the intestinal mucosa of wistar-albino rats after irradiation, and protective effect of ascorbic acid [Wistar-albino sıçanlarda işınlama sonrası İntestinal mukozada oluşan değişiklikler ve askorbik asidin koruyucu etkisi]

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dc.contributor.authorAydin B.
dc.contributor.authorYalman D.
dc.contributor.authorDoğanavşargil B.
dc.contributor.authorKamer S.
dc.contributor.authorEllidokuz H.
dc.date.accessioned2019-10-26T21:30:46Z
dc.date.available2019-10-26T21:30:46Z
dc.date.issued2014
dc.departmentEge Üniversitesien_US
dc.description.abstractObjective: To investigate the histopathological changes and the protective effect of ascorbic acid on the intestinal mucosa of the Wistar-albino rats after irradiation. Material and Methods: Thirty-two young-adult, Wistar albino rats were randomized into control, vitamin C only, radiotherapy only, and radiotherapy + vitamin C groups. Vitamin C (250 mg/kg/day) was added to the drinking water of the rats in vitamin C groups for 10 days. A single dose of 8 Gy radiotherapy was applied to the abdominal region of the rats on the eleventh day. The rats were sacrificed after 72 hours, and their all gastrointestinal tract, from distal esophagus to the anus, was removed. A 1-cm segment selected from a 5-cm proximal part of ilioceacal valve from the ileum and 5 cm distal part from the colon were analyzed regarding crypt count, abnormal epithelial cells, mucosal congestion, edema, cryptic tissue changes, villus height, structural changes, degradation and union of the villi, subepithelial detachment, and mitotic and apoptotic figure counts in the basement membrane. Results: In the radiotherapy only group; crypt count decreased, apoptotic figure count increased, the villi showed extreme degradation, union and shortening, there were lymphocyte infiltration and epithelial cells in the lumen. In the radiotherapy + vitamin C group; apoptotic figure count was very low, villus structure, cryptic and mucosal layer were preserved partially. When radiotherapy only group was compared to radiotherapy + vitamin C group, villus height differed in favor of radiotherapy + vitamin C group (p=0.001), however no differences regarding mitotic count, apoptotic figure and crypt count were found (p=0.03, p=0.524, p=0.772, respectively). Conclusion: Vitamin C protects intestinal mucosa from radiation damage, and ascorbic acid may not reduce the radiation-induced antitumor activity. Prophylactic use of vitamin C in pelvic irradiation can prevent the side effects. However, it could act as a pro-oxidant in higher doses, therefore dose-controlled studies are needed in tumor tissues. © 2014 by Türkiye Klinikleri.en_US
dc.identifier.doi10.5336/medsci.2013-35421
dc.identifier.endpage306en_US
dc.identifier.issn1300-0292
dc.identifier.issn1300-0292en_US
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage298en_US
dc.identifier.urihttps://doi.org/10.5336/medsci.2013-35421
dc.identifier.urihttps://hdl.handle.net/11454/17650
dc.identifier.volume34en_US
dc.indekslendigikaynakScopusen_US
dc.language.isotren_US
dc.publisherTurkiye Kliniklerien_US
dc.relation.ispartofTurkiye Klinikleri Journal of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAscorbic aciden_US
dc.subjectIntestinal mucosaen_US
dc.subjectRadiotherapyen_US
dc.subjectRatsen_US
dc.subjectWistaren_US
dc.titleStructural changes in the intestinal mucosa of wistar-albino rats after irradiation, and protective effect of ascorbic acid [Wistar-albino sıçanlarda işınlama sonrası İntestinal mukozada oluşan değişiklikler ve askorbik asidin koruyucu etkisi]en_US
dc.typeArticleen_US

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