Structural changes in the intestinal mucosa of wistar-albino rats after irradiation, and protective effect of ascorbic acid [Wistar-albino sıçanlarda işınlama sonrası İntestinal mukozada oluşan değişiklikler ve askorbik asidin koruyucu etkisi]
Küçük Resim Yok
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Turkiye Klinikleri
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: To investigate the histopathological changes and the protective effect of ascorbic acid on the intestinal mucosa of the Wistar-albino rats after irradiation. Material and Methods: Thirty-two young-adult, Wistar albino rats were randomized into control, vitamin C only, radiotherapy only, and radiotherapy + vitamin C groups. Vitamin C (250 mg/kg/day) was added to the drinking water of the rats in vitamin C groups for 10 days. A single dose of 8 Gy radiotherapy was applied to the abdominal region of the rats on the eleventh day. The rats were sacrificed after 72 hours, and their all gastrointestinal tract, from distal esophagus to the anus, was removed. A 1-cm segment selected from a 5-cm proximal part of ilioceacal valve from the ileum and 5 cm distal part from the colon were analyzed regarding crypt count, abnormal epithelial cells, mucosal congestion, edema, cryptic tissue changes, villus height, structural changes, degradation and union of the villi, subepithelial detachment, and mitotic and apoptotic figure counts in the basement membrane. Results: In the radiotherapy only group; crypt count decreased, apoptotic figure count increased, the villi showed extreme degradation, union and shortening, there were lymphocyte infiltration and epithelial cells in the lumen. In the radiotherapy + vitamin C group; apoptotic figure count was very low, villus structure, cryptic and mucosal layer were preserved partially. When radiotherapy only group was compared to radiotherapy + vitamin C group, villus height differed in favor of radiotherapy + vitamin C group (p=0.001), however no differences regarding mitotic count, apoptotic figure and crypt count were found (p=0.03, p=0.524, p=0.772, respectively). Conclusion: Vitamin C protects intestinal mucosa from radiation damage, and ascorbic acid may not reduce the radiation-induced antitumor activity. Prophylactic use of vitamin C in pelvic irradiation can prevent the side effects. However, it could act as a pro-oxidant in higher doses, therefore dose-controlled studies are needed in tumor tissues. © 2014 by Türkiye Klinikleri.
Açıklama
Anahtar Kelimeler
Ascorbic acid, Intestinal mucosa, Radiotherapy, Rats, Wistar
Kaynak
Turkiye Klinikleri Journal of Medical Sciences
WoS Q Değeri
Scopus Q Değeri
Q4
Cilt
34
Sayı
3
