Isolation, Identification, and Antimicrobial Evaluation of Secondary Metabolite from Serratia marcescens via an In Vivo Epicutaneous Infection Model

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dc.authoridUyanikgil, Yigit/0000-0002-4016-0522
dc.authoridUyanikgil, Yigit/0000-0002-4016-0522
dc.authoridUyanikgil, Yigit/0000-0002-4016-0522
dc.authoridGökhan, Aylin/0000-0002-6254-157X
dc.authoridUyanikgil, Yigit/0000-0002-4016-0522
dc.authoridSoylu, Fahri Emrah/0000-0002-8405-5468
dc.contributor.authorKarayildirim, Cinel Koksal
dc.contributor.authorSahiner, Asli
dc.contributor.authorCaliskan, Sennur
dc.contributor.authorSoylu, Fahri Emrah
dc.contributor.authorGokhan, Aylin
dc.contributor.authorEroglu, Ebru
dc.contributor.authorUyanikgil, Yigit
dc.date.accessioned2024-08-31T07:50:11Z
dc.date.available2024-08-31T07:50:11Z
dc.date.issued2024
dc.departmentEge Üniversitesien_US
dc.description.abstractMicrobial secondary metabolites, which play a pivotal role in struggling with infectious diseases, are the new source for controlling bacterial contaminations and possess a strong antimicrobial potential. The present study is designed to evaluate the in vitro and in vivo bactericidal activities of prodigiosin against Staphylococcus aureus. For this purpose, Serratia marcescens was used to produce prodigiosin. Characterization of the prodigiosin was carried out using NMR. In addition, bioautographic detection of prodigiosin was detected by TLC. Antibacterial assays, in vivo epicutaneous infection tests, swap analyses, and histopathological examinations were determined. The results revealed that prodigiosin was detected by NMR and TLC. According to antimicrobial susceptibility tests, prodigiosin is an efficient bactericidal compound that demonstrated strong antibacterial activity toward S. aureus. In vivo, animal studies determined that the strong inhibition of S. aureus-caused epidermal infection occurs by prodigiosin at 48 h. Histopathological results showed that S. aureus + prodigiosin skin sections consist of improved and healthy tissues without any infection area compared with the S. aureus and control groups. The in vivo study verified the antibacterial results with swap analyses, and histopathological findings showed that prodigiosin is a promising microbial metabolite effective against S. aureus infection. This study proved that prodigiosin with excellent bioactivity exhibited antibacterial properties, which might possess massive potential for new therapeutic approaches using micro-organisms.en_US
dc.identifier.doi10.1021/acsomega.3c09522
dc.identifier.endpage8404en_US
dc.identifier.issn2470-1343
dc.identifier.issue7en_US
dc.identifier.pmid38405438en_US
dc.identifier.scopus2-s2.0-85185264694en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage8397en_US
dc.identifier.urihttps://doi.org/10.1021/acsomega.3c09522
dc.identifier.urihttps://hdl.handle.net/11454/105134
dc.identifier.volume9en_US
dc.identifier.wosWOS:001173797500001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmer Chemical Socen_US
dc.relation.ispartofACS Omegaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240831_Uen_US
dc.subjectProdigiosinen_US
dc.subjectPurificationen_US
dc.subjectExtractionen_US
dc.titleIsolation, Identification, and Antimicrobial Evaluation of Secondary Metabolite from Serratia marcescens via an In Vivo Epicutaneous Infection Modelen_US
dc.typeArticleen_US

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