An optimization approach to scale up keratinase production byStreptomyces sp. 2M21 by utilizing chicken feather

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dc.contributor.authorDemir T.
dc.contributor.authorHameş E.E.
dc.contributor.authorÖncel S.S.
dc.contributor.authorVardar-Sukan F.
dc.date.accessioned2019-10-26T21:21:32Z
dc.date.available2019-10-26T21:21:32Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractThe aim of this study was to optimize the culture conditions for keratinase production by a new isolate, Streptomyces sp. 2M21 utilizing chicken feather as an alternative low-cost substrate. Contribution of different independent fermentation variables on keratinase activity was investigated by Plackett-Burman design. Parameters that have the highest contribution were selected for the optimization experiments which were carried out by using response surface methodology (RSM). Relative and mutual effects of fermentation variables on keratinase activity were analyzed with "23 Full Factorial Central Composite Design". Cultivation processes were implemented in 250ml Erlenmeyer flasks containing 50ml medium. Maximum keratinase activity was obtained under optimal conditions of 28°C, 5.0gl-1 chicken feather and 5.5 days of process time. As a result of the optimization studies, a 15 fold increase in keratinase activity was reached compared to the unoptimized conditions. Furthermore, similar results were obtained in the 2l bioreactor under optimized conditions. © 2015 Elsevier Ltd.en_US
dc.description.sponsorshipMUH.005 MAG-112M250en_US
dc.description.sponsorshipThis work was supported by The Research Fund of Ege University (Grant number 11.MUH.005 ) and supported in part by TUBITAK (The Scientific and Technical Research Council of Turkey) (Grant number MAG-112M250 ). --en_US
dc.identifier.doi10.1016/j.ibiod.2015.04.025
dc.identifier.endpage140en_US
dc.identifier.issn0964-8305
dc.identifier.issn0964-8305en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage134en_US
dc.identifier.urihttps://doi.org/10.1016/j.ibiod.2015.04.025
dc.identifier.urihttps://hdl.handle.net/11454/16881
dc.identifier.volume103en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Ltden_US
dc.relation.ispartofInternational Biodeterioration and Biodegradationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEnvironmental biotechnologyen_US
dc.subjectExperimental designen_US
dc.subjectFeatheren_US
dc.subjectFermentationen_US
dc.subjectKeratinaseen_US
dc.subjectOptimizationen_US
dc.titleAn optimization approach to scale up keratinase production byStreptomyces sp. 2M21 by utilizing chicken featheren_US
dc.typeArticleen_US

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