An optimization approach to scale up keratinase production byStreptomyces sp. 2M21 by utilizing chicken feather
Küçük Resim Yok
Tarih
2015
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The aim of this study was to optimize the culture conditions for keratinase production by a new isolate, Streptomyces sp. 2M21 utilizing chicken feather as an alternative low-cost substrate. Contribution of different independent fermentation variables on keratinase activity was investigated by Plackett-Burman design. Parameters that have the highest contribution were selected for the optimization experiments which were carried out by using response surface methodology (RSM). Relative and mutual effects of fermentation variables on keratinase activity were analyzed with "23 Full Factorial Central Composite Design". Cultivation processes were implemented in 250ml Erlenmeyer flasks containing 50ml medium. Maximum keratinase activity was obtained under optimal conditions of 28°C, 5.0gl-1 chicken feather and 5.5 days of process time. As a result of the optimization studies, a 15 fold increase in keratinase activity was reached compared to the unoptimized conditions. Furthermore, similar results were obtained in the 2l bioreactor under optimized conditions. © 2015 Elsevier Ltd.
Açıklama
Anahtar Kelimeler
Environmental biotechnology, Experimental design, Feather, Fermentation, Keratinase, Optimization
Kaynak
International Biodeterioration and Biodegradation
WoS Q Değeri
Scopus Q Değeri
Q1
Cilt
103
