Molecular cytogenetic characterization of common bean (Phaseolus vulgaris L.) accessions

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dc.contributor.authorAşçıoğul, Tansel Kaygısız
dc.contributor.authorEşiyok, Dursun
dc.contributor.authorTuna, Metin
dc.contributor.authorTuna, Gülsemin Savaş
dc.contributor.authorYücel, Gülru
dc.contributor.authorTanyolaç, Muhammed Bahattin
dc.contributor.authorAteş, Duygu
dc.date.accessioned2023-01-12T20:36:56Z
dc.date.available2023-01-12T20:36:56Z
dc.date.issued2020
dc.departmentN/A/Departmenten_US
dc.description.abstractWith an annual global production of approximately 25 million tons, the common bean (Phaseolus vulgaris L), a member of the genus Phaseolus, is one of the major protein sources used as food for humans. In this study, it was aimed to investigate the genome size of the common bean genetic resource collection (154 common bean accessions) in Turkey by flow cytometry (FCM) and determine whether geographical variables affected the genome size. In addition, the number and distribution of 5S and 45S ribosomal DNA loci were designated by performing a fluorescence in situ hybridization (FISH) analysis in some of the accessions. The FCM analyses revealed that the mean nuclear DNA content of the accessions varied from 1.28 pg2C-1 to 1.55 pg2G1 (mean 1.35 pg2C-1), and the differences between these accessions were statistically significant (P < 0.01). Intraspecific variation in the genome size was determined, and a positive correlation was found between the altitude and genome size. However, latitude and longitude did not have any statistically significant effect on the genome size. In the principal coordinate analysis, the accessions were divided into 3groups. Based on the results of the FISH analysis performed on 5 different accessions with varying genome sizes, using 5S and 45S rDNA genes as probes, the number of 5S rDNA loci was 4 in the common bean and stable among the common bean accessions, while the number of 45S rDNA loci was highly polymorphic, varying between 6 and 16. Consequently, it was determined in the present study that the genetic resource collection of common bean had a wide variation in terms of genome size and genome organization.en_US
dc.identifier.endpage630en_US
dc.identifier.issn1303-6173
dc.identifier.issue6en_US
dc.identifier.startpage612en_US
dc.identifier.trdizinid433291en_US
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/433291
dc.identifier.urihttps://hdl.handle.net/11454/81502
dc.identifier.volume44en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Agriculture and Forestryen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectflow cytometryen_US
dc.subjectgene poolen_US
dc.subjectgenome sizeen_US
dc.subjectgeographical variablesen_US
dc.subjectfluorescence in situ hybridizationen_US
dc.subjectPhaseolus vulgarisen_US
dc.titleMolecular cytogenetic characterization of common bean (Phaseolus vulgaris L.) accessionsen_US
dc.typeArticleen_US

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