Green Synthesis, Optimization, and Characterization of Silver Nanoparticles from Euphorbia rigida Leaf Extract and Investigation of Their Antimicrobal Activities

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Tarih

2021

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Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

Green synthesis is a biological method known for being environment-friendly, cheap and for retrieval of nanoparticles with ease, which is preferred more than chemical and physical methods. Various metallic nanoparticles with potent bioactivities have been synthesized using plant extracts. In this study, synthesis of silver nanoparticles (AgNPs) using the aqueous leaf extract of Euphorbia rigida was investigated. Their antibacterial activity was determined using microdilution methods. The optimization of the synthesis was performed via the adjustment of different parameters including pH, temperature, concentration of the plant extract, the different concentrations of AgNO3, and synthesis time using UVvisible spectroscopy. Silver nanoparticles (Er-AgNPs) that were synthesized by Euphorbia rigida characterized by Dynamic Light Scattering (DLS) and Scanning Electron Microscope (SEM) analyses and their antimicrobial potential were tested on six different pathogens. The synthesis process resulted in spherical-shaped NPs with a size ranging between 50-75nm. The AgNPs demonstrated a strong antimicrobial activity against various pathogens (Escherichia coli, Staphylococcus aureus, Enterococcus faecium, Staphylococcus epidermidis, Salmonella enteritidis, Salmonella typhimurium, Listeria monocytogenes and Candida albicans). The results showed that the synthesized Er-AgNPs can be used as a biological material for biomedical, pharmaceutical, biotechnology and industrial areas. In this study, the antimicrobial potential of silver nanoparticles which are prepared using Euphorbia rigida leaf extract isreported for the first time.

Açıklama

Anahtar Kelimeler

Antimicrobial Activity, Dynamic Light Scattering, Euporbia Rigida, Scanning Electron Microscope, Silver Nanoparticle Antimikrobiyal Aktivite, Dinamik Işık Saçılımı, Euporbia Rigida, Taramalı Elektron Mikroskobu, Gümüş Nanopartikül

Kaynak

Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi

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Cilt

8

Sayı

2

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