Fecal calprotectin as a factor that supports the pathogenicity of Dientamoeba fragilis

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dc.contributor.authorAykur, Mehmet
dc.contributor.authorArmagan, Guliz
dc.contributor.authorVardar, Rukiye
dc.contributor.authorDagci, Hande
dc.date.accessioned2020-12-01T12:02:41Z
dc.date.available2020-12-01T12:02:41Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractCalprotectin is a protein that is mostly released from neutrophils, monocytes, macrophages and submucosal epithelial cells. Fecal calprotectin (f-CP) is a marker of intestinal inflammation. There are some discussions about the pathogenicity of D. fragilis in the gastrointestinal tract. in this study, we investigated whether f-CP level is a factor supporting the pathogenicity of D. fragilis. the f-CP levels were evaluated in patients with only D. fragilis positive in comparison with healthy controls. Moreover, the levels of f-CP were investigated in fecal samples of D. fragilis negative patients with gastrointestinal complaints. the fecal samples were collected from three groups. Three groups of fecal samples were examined directly microscopy, trichrome staining, cultivation, enzyme immunoassay (EIA) and real-time PCR assay. in the first group (Group 1, n = 34), patient stool samples with gastrointestinal symptoms (without other pathogens) found only with D. fragilis were included. in the second group (Group 2, n = 31), there were patients' stool samples with gastrointestinal symptoms that D. fragilis was negative (but there may be other pathogenic agents). in the control group (Group 3, n = 23), we used fecal samples collected from healthy volunteers without any infection or gastrointestinal complaints. the collected fecal samples were stored at -20 degrees C until analysis. Levels of f-CP were determined by using human calprotectin ELISA kits. Total of 88 patients were enrolled in three different groups. We obtained f-CP levels as follows: 33.40 ng/mg protein in the group 1, 15.99 ng/mg protein in the group 2 and 1.54 ng/mg protein in the group 3. Statistically significant difference in f-CP levels of the group 1 and the group 2 were obtained when compared with healthy controls (p < 0.0001). However, the f-CP levels of the group 1 were not significantly different from the group 2 (p > 0.99). in conclusion, increased levels of f-CP are shown as a marker of an inflammatory disease of the lower gastrointestinal tract in infected humans. There is continues controversy about the pathogenicity of D. fragilis in symptomatic and asymptomatic patients. the findings of this study contribute to the ongoing debate about the pathogenicity of D. fragilis. in our study, the potential pathogenicity of D. fragilis is associated with increased f-CP concentrations with parasite detection in the fecal samples and therefore we assume that the parasite is not only a harmless commensal. in summary, higher levels of f-CP found in D. fragilis positive patients suggest the importance of researches that support the pathogenicity of indicated parasite.en_US
dc.description.sponsorshipScientific Research Projects Branch Directorate of Ege University, TurkeyEge University [13-TIP-092]en_US
dc.description.sponsorshipThis study was partly supported by the grant given by the Scientific Research Projects Branch Directorate of Ege University, Turkey (Grant number: 13-TIP-092).en_US
dc.identifier.doi10.1016/j.micpath.2019.103868en_US
dc.identifier.issn0882-4010
dc.identifier.pmid31730996en_US
dc.identifier.scopus2-s2.0-85074954261en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttps://doi.org/10.1016/j.micpath.2019.103868
dc.identifier.urihttps://hdl.handle.net/11454/62737
dc.identifier.volume139en_US
dc.identifier.wosWOS:000514245100013en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAcademic Press Ltd- Elsevier Science Ltden_US
dc.relation.ispartofMicrobial Pathogenesisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDientamoeba fragilisen_US
dc.subjectFecal calprotectinen_US
dc.subjectPathogenicityen_US
dc.subjectGastrointestinal symptomsen_US
dc.titleFecal calprotectin as a factor that supports the pathogenicity of Dientamoeba fragilisen_US
dc.typeArticleen_US

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