Synthesis and characterization of cryogel structures for isolation of EPSs from Botryococcus braunii

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dc.contributor.authorTuru, Irem Cemre
dc.contributor.authorTurkcan-Kayhan, Ceren
dc.contributor.authorKazan, Aslihan
dc.contributor.authorYildiz-Ozturk, Ece
dc.contributor.authorAkgol, Sinan
dc.contributor.authorYesil-Celiktas, Ozlem
dc.date.accessioned2019-10-27T11:25:23Z
dc.date.available2019-10-27T11:25:23Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractIn this study, the objective was to separate exopolysaccharides (EPSs) released in the broth subsequent to outdoor cultivation of Botryococcus braunii. For this, poly(2-hydroxyethyl methacrylate) (PHEMA) cryogels were synthesized. After that, the surface was modified by coupling Concanavalin A. Box-Behnken statistical design was used to evaluate the effect of freezing temperature, Con A concentration and flow rate on Con A binding capacity. Optimum synthesis conditions were elicited as -14.48 degrees C freezing temperature, 1.00 mg/ml Con A concentration and 0.30 ml/min flow rate yielding 3.18 mg Con A/g cryogel, whereas -16 degrees C, 1.00 mg/ml and 0.30 ml/min yielded the highest (3.38 mg) binding capacity in experimental cryogel preparation. The EPS adsorption capacity of the optimum cryogel column was found as 3.26 mg EPS/g cryogel corresponding to adsorption yield of 80%. Besides; swelling test, elemental analysis, Micro-CT, SEM and FTIR analysis were carried out for characterization of the synthesized cryogels. (C) 2016 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipEUEuropean Union (EU) [311956]en_US
dc.description.sponsorshipThis work is financially supported by EU FP7 SPLASH Project (311956).en_US
dc.identifier.doi10.1016/j.carbpol.2016.05.027en_US
dc.identifier.endpage384en_US
dc.identifier.issn0144-8617
dc.identifier.issn1879-1344
dc.identifier.pmid27312648en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage378en_US
dc.identifier.urihttps://doi.org/10.1016/j.carbpol.2016.05.027
dc.identifier.urihttps://hdl.handle.net/11454/33382
dc.identifier.volume150en_US
dc.identifier.wosWOS:000377971800043en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofCarbohydrate Polymersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCryogelen_US
dc.subjectExopolysaccharidesen_US
dc.subjectB. brauniien_US
dc.subjectSynthesisen_US
dc.subjectPHEMAen_US
dc.titleSynthesis and characterization of cryogel structures for isolation of EPSs from Botryococcus brauniien_US
dc.typeArticleen_US

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