Simple workflow to repurpose SARS-CoV-2 swab/serum samples for the isolation of cost-effective antibody/antigens for proteotyping applications and diagnosis

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dc.authoridTimur, Suna/0000-0002-1981-7577
dc.authoridMOULAHOUM, Hichem/0000-0003-3934-6415
dc.authoridGhorbanizamani, Faezeh/0000-0001-8541-0540
dc.authoridBALABAN HANOGLU, Simge/0000-0002-4540-1882
dc.authoridTOK, Kerem/0000-0002-7244-3949
dc.authoridDurmus, Ceren/0000-0002-8648-0505
dc.authorscopusid57220178117
dc.authorscopusid57188963997
dc.authorscopusid57199103659
dc.authorscopusid55935858800
dc.authorscopusid57289928300
dc.authorscopusid57210729367
dc.authorscopusid8558322700
dc.authorwosidÇiçek, Candan/ABH-1161-2021
dc.authorwosidEvran, Serap/AFU-1537-2022
dc.authorwosidTimur, Suna/CAG-0914-2022
dc.authorwosidTok, Kerem/AAG-6529-2021
dc.authorwosidMOULAHOUM, Hichem/DIC-3335-2022
dc.authorwosidGhorbanizamani, Faezeh/ABE-3800-2021
dc.contributor.authorTok, Kerem
dc.contributor.authorMoulahoum, Hichem
dc.contributor.authorGhorbanizamani, Faezeh
dc.contributor.authorHarmanci, Duygu
dc.contributor.authorHanoglu, Simge Balaban
dc.contributor.authorDurmus, Ceren
dc.contributor.authorEvran, Serap
dc.date.accessioned2023-01-12T19:50:04Z
dc.date.available2023-01-12T19:50:04Z
dc.date.issued2021
dc.departmentN/A/Departmenten_US
dc.description.abstractSupply shortage for the development and production of preventive, therapeutic, and diagnosis tools during the COVID-19 pandemic is an important issue affecting the wealthy and poor nations alike. Antibodies and antigens are especially needed for the production of immunological-based testing tools such as point-of-care tests. Here, we propose a simple and quick magnetic nanoparticle (MNP)-based separation/isolation approach for the repurposing of infected human samples to produce specific antibodies and antigen cocktails. Initially, an antibody cocktail was purified from serums via precipitation and immunoaffinity chromatography. Purified antibodies were conjugated onto MNPs and used as an affinity matrix to separate antigens. The characterization process was performed by ELISA, SDS-PAGE, electrochemistry, isothermal titration calorimetry, and LC-Q-TOF-MS/MS analyses. The MNP-separated peptides can be used for mass spectrometry-based as well as paper-based lateral flow assay diagnostic. The exploitation of the current workflow for the development of efficient diagnostic tools, specific treatments, and fundamental research can significantly impact the present or eventual pandemic. This workflow can be considered as a two birds, one stone-like strategy.en_US
dc.description.sponsorshipTUBITAK [120R021]; Republic of Turkey Ministry of Development [2010K120810/2020K12150700, 2016K121190]en_US
dc.description.sponsorshipThe study was supported by TUBITAK (Project number: 120R021). The Republic of Turkey Ministry of Development provided the support for the infrastructure of EGEMATAL (Ege University/Izmir) via 2010K120810/2020K12150700 and 2016K121190 grants.en_US
dc.identifier.doi10.1007/s00216-021-03654-4
dc.identifier.endpage7263en_US
dc.identifier.issn1618-2642
dc.identifier.issn1618-2650
dc.identifier.issue29en_US
dc.identifier.pmid34622322en_US
dc.identifier.scopus2-s2.0-85116740955en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage7251en_US
dc.identifier.urihttps://doi.org/10.1007/s00216-021-03654-4
dc.identifier.urihttps://hdl.handle.net/11454/76005
dc.identifier.volume413en_US
dc.identifier.wosWOS:000704938900002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer Heidelbergen_US
dc.relation.ispartofAnalytical and Bioanalytical Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSARS-CoV-2en_US
dc.subjectAntigenen_US
dc.subjectantibody purificationen_US
dc.subjectMagnetic nanoparticle (MNP)-based separationen_US
dc.subjectMass spectrometryen_US
dc.subjectLateral flow assayen_US
dc.subjectMagnetic Nanoparticlesen_US
dc.subjectAntibodiesen_US
dc.subjectPurificationen_US
dc.subjectInformationen_US
dc.subjectCleavageen_US
dc.titleSimple workflow to repurpose SARS-CoV-2 swab/serum samples for the isolation of cost-effective antibody/antigens for proteotyping applications and diagnosisen_US
dc.typeArticleen_US

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