Molecular prevalence of Blastocystis sp. and subtype diversity in fecal samples collected from cattle in dairy farms in Turkey

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dc.authoridCan, Hüseyin/0000-0001-9633-9786
dc.authoridÖncü Öner, Tülay/0000-0001-6180-5409
dc.authoridkarakavuk, muhammet/0000-0002-2468-5564
dc.authoridKoseoglu, Ahmet Efe/0000-0002-3505-4397
dc.authorscopusid57204833497
dc.authorscopusid56495590200
dc.authorscopusid57201674137
dc.authorscopusid57221117593
dc.authorscopusid57219922088
dc.authorscopusid25825299300
dc.authorscopusid57212213434
dc.authorwosidDegirmenci, Aysu/DET-8805-2022
dc.authorwosidCan, Hüseyin/AAG-3152-2020
dc.authorwosidErkunt Alak, Sedef/AAG-9556-2020
dc.authorwosidÖncü Öner, Tülay/HDL-9506-2022
dc.authorwosidkarakavuk, muhammet/ABC-8038-2020
dc.contributor.authorOner, Tuelay Oncu
dc.contributor.authorKarakavuk, Muhammet
dc.contributor.authorDoskaya, Aysu Degirmenci
dc.contributor.authorGuvendi, Mervenur
dc.contributor.authorGuel, Aytul
dc.contributor.authorKoseoglu, Ahmet Efe
dc.contributor.authorAlak, Sedef Erkunt
dc.date.accessioned2023-01-12T19:54:35Z
dc.date.available2023-01-12T19:54:35Z
dc.date.issued2022
dc.departmentN/A/Departmenten_US
dc.description.abstractClose contact with infected animals is one of the main risk factors for zoonotic transmission of enteric protozoan parasite Blastocystis and thus, several animal species are being screened for the detection of the zoonotic subtypes. For this purpose, 22 fecal samples were collected from healthy cattle aged > 2 months and 39 fecal samples were also collected from cattle (aged <2 months) which are treated with TMP-SMX due to diarrhea. Later, Blastocystis sp. and subtypes were investigated by a PCR targeting the SSU rRNA gene and subsequently by sequencing. Among the 22 fecal samples collected from healthy cattle, Blastocystis was detected in 12 of them with a prevalence rate of 54.5 %. Among Blastocystis-positive samples, five different subtypes (ST3, ST5, ST10, ST12, and ST13) were detected. The predominant subtype was ST10 (allele 152) with a prevalence rate of 50 % (6/12). In the other group treated with TMP-SMX due to diarrhea, Blastocystis was detected in only one (2.56 %;1/39) fecal sample and its subtype was ST1 (allele 2). High prevalence of Blastocystis as well as predominance of ST10 (allele 152) were detected in healthy cattle. The identification of zoonotic ST1, ST3, ST5 and ST12 subtypes among the detected subtypes with a high prevalence (46.1 %; 6/13) showed the importance of cattle as a source for transmission of Blastocystis to humans. It was observed that the efficiency of TMP-SMX on the clearance of Blastocystis in cattle was very strong. Moreover, to our knowledge, this is the first study detecting Blastocystis ST13 subtype in the cattle.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [119S785]; Ege University Scientific Research Projects Coordination Unit [TGA-020-20850]en_US
dc.description.sponsorshipThis study was partially supported by a project provided by The Scientific and Technological Research Council of Turkey (TUBITAK) (Project number: 119S785) to H.C and Ege University Scientific Research Projects Coordination Unit (Project number: TGA-020-20850) to M.K.en_US
dc.identifier.doi10.1016/j.cimid.2022.101850
dc.identifier.issn0147-9571
dc.identifier.issn1878-1667
dc.identifier.pmid35751914en_US
dc.identifier.scopus2-s2.0-85132750038en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.cimid.2022.101850
dc.identifier.urihttps://hdl.handle.net/11454/76468
dc.identifier.volume87en_US
dc.identifier.wosWOS:000818516500007en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofComparative Immunology Microbiology and Infectious Diseasesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBlastocystis spen_US
dc.subjectGenotypesen_US
dc.subjectSequencingen_US
dc.subjectPCRen_US
dc.subjectSSU rRNAen_US
dc.subjectPcr-Based Identificationen_US
dc.subjectGenetic Diversityen_US
dc.subjectZoo Animalsen_US
dc.subjectProvinceen_US
dc.subjectCryptosporidiumen_US
dc.subjectPolymorphismen_US
dc.subjectInfectionsen_US
dc.subjectHumansen_US
dc.subjectPigsen_US
dc.subjectSp.en_US
dc.titleMolecular prevalence of Blastocystis sp. and subtype diversity in fecal samples collected from cattle in dairy farms in Turkeyen_US
dc.typeArticleen_US

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