Application of ?-glucuronidase-immobilised silica gel formulation to microfluidic platform for biotransformation of ?-glucuronides
Küçük Resim Yok
Tarih
2018
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer Netherlands
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Objective: To improve the efficiency of reactions of ?-glucuronidase (GUS)-assisted glucuronic acid (GluA) removal within a microfluidic system. Results: ?-glucuronidase from Helix pomatia was immobilised and characterised in silica-based sol–gel monoliths. Efficiency of the GUS-doped silica monoliths was tested for hydrolysis of p-Nitrophenyl-?-d-glucuronide (pNP–GluA) in both ml-scaled medium via batch reactions and microfluidic environment via continuous-flow reactions. In the microfluidic platform, within a duration of 150 min of continuous operation (flow rate: 1 µL/min), the obtained highest pNP yield was almost 50% higher than that of the corresponding batchwise reaction. However, increased flow rates (3, 5, and 10 µL/min) resulted in lower conversion yields compared to 1 µL/min. The microfluidic platform demonstrated continuous hydrolytic activity for 7 days with considerable reaction yields while using a small amount of the enzyme. Conclusion: These results revealed that usage of the microreactors has considerable potential to efficiently obtain bioactive GluA-free aglycons from various plant-derived ?-glucuronides for pharmaceutical applications. © 2018, Springer Science+Business Media B.V., part of Springer Nature.
Açıklama
Anahtar Kelimeler
Enzyme immobilisation, Glucuronide hydrolysis, Microenzyme reactor, Microfluidic platform, Sol–gel, ?-glucuronidase
Kaynak
Biotechnology Letters
WoS Q Değeri
Scopus Q Değeri
N/A
Cilt
40
Sayı
5
