Medium modification with bone morphogenetic protein 2 addition for odontogenic differentiation

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dc.contributor.authorAtalayin, Cigdem
dc.contributor.authorTezel, Huseyin
dc.contributor.authorDagci, Taner
dc.contributor.authorYavasoglu, Nefise Ulku Karabay
dc.contributor.authorOktem, Gulperi
dc.date.accessioned2019-10-27T22:58:06Z
dc.date.available2019-10-27T22:58:06Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractThe aim of this study was to evaluate whether medium modification improves the odontogenic differentiation of human dental pulp stem cells (DPSC) in vitro and in vivo. DPSC isolated from human impacted third molar teeth were analysed for clusters of differentiation with flow cytometry. Odontogenic differentiation was stimulated by medium modification with the addition of bone morphogenetic protein 2 (BMP2). The expression of dentin sialophosphoprotein, dentin matrix protein 1, enamelysin/matrix metalloproteinase 20 and the phosphate-regulating gene with homologies to endopeptidases on the X chromosome of the cells were analysed with RT-PCR at 7, 14 and 21 days. Then, DPSC were transplanted on the back of immunocompromised mice via a hydroxyapatite tricalcium phosphate scaffold, and the structure of the formed tissue was investigated. The cells were identified as mesenchymal stem cells with a 98.3% CD73 and CD90 double-positive cell rate. The increase in mineralization capacity and expression of human enamel-dentin specific transcripts proportional to the culture period were determined after differentiation. Six weeks after transplantation, an osteo-dentin matrix was formed in the group in which odontogenic differentiation was stimulated, and the odontogenic characteristics of the matrix were confirmed by histological examination and RT-PCR analysis. Odontogenic differentiation of the isolated and characterized human DPSC was improved with medium modification by the addition of BMP2 in vitro and in vivo. The defined medium and applied technique have a potential use for forming reparative dentin in the future, but the effects of the method should be investigated in long-term studies.en_US
dc.description.sponsorshipEge University's Scientific Research FoundationEge University [2009-Dis-036]; Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)en_US
dc.description.sponsorshipThis study was supported by Ege University's Scientific Research Foundation (2009-Dis-036). We also acknowledge The Scientific and Technological Research Council of Turkey (TUBITAK) for its PhD scholarship support.en_US
dc.identifier.doi10.1590/1807-3107BOR-2016.vol30.0020en_US
dc.identifier.issn1806-8324
dc.identifier.issn1678-8079
dc.identifier.issue1en_US
dc.identifier.pmid26981753en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttps://doi.org/10.1590/1807-3107BOR-2016.vol30.0020
dc.identifier.urihttps://hdl.handle.net/11454/51439
dc.identifier.volume30en_US
dc.identifier.wosWOS:000378324000020en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSociedade Brasileira De Pesquisa Odontologicaen_US
dc.relation.ispartofBrazilian Oral Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBone Morphogenetic Protein 2en_US
dc.subjectDental Pulpen_US
dc.subjectRegenerationen_US
dc.titleMedium modification with bone morphogenetic protein 2 addition for odontogenic differentiationen_US
dc.typeArticleen_US

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