DEVELOPMENT OF GENETICALLY ENCODED FLUORESCENT PROTEIN CONSTRUCTS OF HYPERTHERMOPHILIC MALTOSE-BINDING PROTEIN

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dc.contributor.authorOzyurt, Canan
dc.contributor.authorEvran, Serap
dc.contributor.authorTelefoncu, Azmi
dc.date.accessioned2019-10-27T22:05:19Z
dc.date.available2019-10-27T22:05:19Z
dc.date.issued2014
dc.departmentEge Üniversitesien_US
dc.description.abstractCircularly permuted green fluorescent protein (cGFP) was inserted into the hyperthermophilic maltose binding protein at two different locations. cGFP was inserted between amino acid residues 206 and 207, or fused to the N-terminal of maltose binding protein from Thermotoga maritima. The cloned DNA constructs were expressed in Escherichia coli cells, and purified by metal chelate affinity chromatography. Conformational change upon ligand binding was monitored by the increase in fluorescence intensity. Both of the fusion proteins developed significant fluorescence change at 0.5mM maltose concentration, whereas their maltose binding affinities and optimum incubation times were different. Fluorescent biosensors based on mesophilic maltose binding proteins have been described in the literature, but there is a growing interest in biosensors based on thermostable proteins. Therefore, the developed protein constructs could be models for thermophilic protein-based fluorescent biosensors.en_US
dc.description.sponsorshipResearch Foundation of Ege UniversityEge University [11FEN035]en_US
dc.description.sponsorshipThe authors thank Prof. Dr. Konstantinos Vorgias (Athens University, Greece) for generously supplying the genomic DNA of T. maritima. This work was supported by the Research Foundation of Ege University (project 11FEN035).en_US
dc.identifier.doi10.1080/10826068.2013.797436en_US
dc.identifier.endpage145en_US
dc.identifier.issn1082-6068
dc.identifier.issn1532-2297
dc.identifier.issue2en_US
dc.identifier.pmid24152100en_US
dc.identifier.startpage132en_US
dc.identifier.urihttps://doi.org/10.1080/10826068.2013.797436
dc.identifier.urihttps://hdl.handle.net/11454/48351
dc.identifier.volume44en_US
dc.identifier.wosWOS:000325959600003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofPreparative Biochemistry & Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcircular permutationen_US
dc.subjectgreen fluorescent proteinen_US
dc.subjectmaltoseen_US
dc.subjectperiplasmic ligand binding proteinen_US
dc.subjectpurificationen_US
dc.subjectThermotoga maritimaen_US
dc.titleDEVELOPMENT OF GENETICALLY ENCODED FLUORESCENT PROTEIN CONSTRUCTS OF HYPERTHERMOPHILIC MALTOSE-BINDING PROTEINen_US
dc.typeArticleen_US

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