Contribution of Gamma amino butyric acid (GABA) to salt stress responses of Nicotiana sylvestris CMSII mutant and wild type plants

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dc.contributor.authorAkcay, Nese
dc.contributor.authorBor, Melike
dc.contributor.authorKarabudak, Tugba
dc.contributor.authorOzdemir, Filiz
dc.contributor.authorTurkan, Ismail
dc.date.accessioned2019-10-27T21:43:49Z
dc.date.available2019-10-27T21:43:49Z
dc.date.issued2012
dc.departmentEge Üniversitesien_US
dc.description.abstractPlants accumulate high levels of Gamma amino butyric acid (GABA) in response to different environmental stresses and GABA metabolism has different functions such as osmotic and pH regulation, bypass of tricarboxylic acid cycle, and C:N balance. The cytoplasmic male sterile (CMS) II mutant of Nicotiana sylvestris has a deletion in the mitochondrial gene nad7 which encodes the NAD7 subunit of complex I which causes increased leaf respiration, impaired photosynthesis, slower growth and increased amino acid levels. In this study we aimed to elucidate the role of GABA and GABA metabolism in different genotypes of the same plant system under salt stress (100 mM NaCl) in short (24h) and long (7, 14 and 21 days) terms. We have investigated the differences in leaf fresh and dry weights, relative water content, photosynthetic efficiency (F-v/F-m), glutamate dehydrogenase (GDH. EC 1.4.1.4) and glutamate decarboxylase (GAD, EC 4.1.1.15) enzyme activities, GABA content and GAD gene expression profiles. GDH activity showed variations in CMSII and wild type (WT) plants in the first 24 h. GAD gene expression profiles were in good agreement with the GAD enzyme activity levels in CMSII and WT plants after 24 h. In long-term salinity, GAD activities increased in \NT but, decreased in CMSII. GABA accumulation in WT and CMSII plants in short and long term was induced by salt stress. Variations in GDH and GAD activities in relation to GABA levels were discussed and GABA metabolism has been proposed to be involved in better performance of CMSII plants under long term salinity. (C) 2011 Elsevier GmbH. All rights reserved.en_US
dc.description.sponsorshipEge University Research FoundationEge University [2009-FEN-020]en_US
dc.description.sponsorshipWe wish to thank Dr. Rosine De Paepe (Institut de Biotechnologie des Plantes, Orsay, France) for providing us N. sylvestris CMSII mutant and WT seeds. This work was supported by grant-in-aids 2009-FEN-020 from Ege University Research Foundation.en_US
dc.identifier.doi10.1016/j.jplph.2011.11.006en_US
dc.identifier.endpage458en_US
dc.identifier.issn0176-1617
dc.identifier.issn1618-1328
dc.identifier.issue5en_US
dc.identifier.pmid22189426en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage452en_US
dc.identifier.urihttps://doi.org/10.1016/j.jplph.2011.11.006
dc.identifier.urihttps://hdl.handle.net/11454/47151
dc.identifier.volume169en_US
dc.identifier.wosWOS:000302495000003en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Gmbhen_US
dc.relation.ispartofJournal of Plant Physiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGAMen_US
dc.subjectGABA shunten_US
dc.subjectSalt stressen_US
dc.subjectTobaccoen_US
dc.titleContribution of Gamma amino butyric acid (GABA) to salt stress responses of Nicotiana sylvestris CMSII mutant and wild type plantsen_US
dc.typeArticleen_US

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