Gingival crevicular fluid can degrade Emdogain and inhibit Emdogain-induced proliferation of periodontal ligament fibroblasts

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dc.contributor.authorLaaksonen, M.
dc.contributor.authorSalo, T.
dc.contributor.authorVardar-Sengul, S.
dc.contributor.authorAtilla, G.
dc.contributor.authorSaygan, B. Han
dc.contributor.authorSimmer, J. P.
dc.contributor.authorBaylas, H.
dc.contributor.authorSorsa, T.
dc.date.accessioned2019-10-27T21:17:28Z
dc.date.available2019-10-27T21:17:28Z
dc.date.issued2010
dc.departmentEge Üniversitesien_US
dc.description.abstractBackground and Objective: Emdogain (R) (EMD), consisting mostly of amelogenin, is used in periodontal therapy to regenerate lost connective tissue. Emdogain is applied onto periodontally affected root surfaces, where it becomes exposed to proteolytic enzymes. In this study, we aimed to find out whether gingival crevicular fluid or matrix metalloproteinases (MMPs) could degrade EMD, and whether this degradation has consequences for in vitro cell proliferation. Material and Methods: We studied the effects of 156 gingival crevicular fluid samples collected from subjects with different stages of periodontal disease and from healthy control subjects and the effects of MMP-1, -2, -8, -9, -13 and -14 on the degradation of EMD using EMD-embedded zymography. The effects of gingival crevicular fluid with or without EMD and the effects of amelogenin on the proliferation of cultured periodontal ligament fibroblasts were studied by cell proliferation enzyme-linked immunosorbent assay kit. Results: Degradation of Emdogain induced by gingival crevicular fluid was greater in samples from all stages of periodontal diseases compared with healthy control samples. Of the MMPs studied, only MMP-2 and MMP-8 showed limited EMD-degrading activities. One hundred micrograms per millilitre of EMD increased proliferation of periodontal ligament fibroblasts on average by 24% (confidence interval 0.60-0.64) and at 200 mu g/mL by 30% (confidence interval 0.62-0.68) compared with control fibroblasts (confidence interval 0.48-0.52). However, gingival crevicular fluid (10 mu g/mL) together with 100 mu g/mL EMD induced the proliferation only by 6% (confidence interval 0.51-0.55) and with 200 mu g/mL EMD by 12% (confidence interval 0.54-0.58). Amelogenin at 200 mu g/mL decreased the proliferation of periodontal ligament fibroblasts by 54% (confidence interval 0.22-0.25). Conclusion: We suggest that diseased gingival crevicular fluid containing various proteases leads to degradation of EMD and decreased proliferation of periodontal ligament fibroblasts.en_US
dc.description.sponsorshipAcademy of FinlandAcademy of Finland; Helsinki University Central Hospital Research Foundation; Oulu University Central Hospital Research Foundation; Finnish Dental Society Apolloniaen_US
dc.description.sponsorshipThe skilful technical assistance of Ms Maija-Leena Lehtonen (at the University of Oulu) is greatly appreciated. This study was supported by grants from the Academy of Finland, the Helsinki University Central Hospital Research Foundation, the Oulu University Central Hospital Research Foundation and the Finnish Dental Society Apollonia. We ensure that none of the authors have any financial or personal relationships with other people or organizations that could inappropriately influence our work.en_US
dc.identifier.doi10.1111/j.1600-0765.2009.01244.xen_US
dc.identifier.endpage360en_US
dc.identifier.issn0022-3484
dc.identifier.issn1600-0765
dc.identifier.issue3en_US
dc.identifier.pmid19909398en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage353en_US
dc.identifier.urihttps://doi.org/10.1111/j.1600-0765.2009.01244.x
dc.identifier.urihttps://hdl.handle.net/11454/43823
dc.identifier.volume45en_US
dc.identifier.wosWOS:000276699300008en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWiley-Blackwellen_US
dc.relation.ispartofJournal of Periodontal Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEmdogainen_US
dc.subjectamelogeninen_US
dc.subjectgingival crevicular fluiden_US
dc.subjectperiodontitisen_US
dc.subjectproliferationen_US
dc.subjectmatrix metalloproteinaseen_US
dc.titleGingival crevicular fluid can degrade Emdogain and inhibit Emdogain-induced proliferation of periodontal ligament fibroblastsen_US
dc.typeArticleen_US

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