Different mRNA and Protein Expression of Versican in TGF-?1- treated Prostate Cancer Cells

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dc.contributor.authorTaskiran, Aysegul
dc.contributor.authorAçıkgöz, Eda
dc.contributor.authorSoner, Burak Cem
dc.contributor.authorÖktem, Gülperi
dc.contributor.authorCaggia, Silvia
dc.contributor.authorKhan, Shafıq
dc.date.accessioned2024-08-25T18:51:52Z
dc.date.available2024-08-25T18:51:52Z
dc.date.issued2022
dc.departmentEge Üniversitesien_US
dc.description.abstractObjective: Cellular heterogeneity of tumors stems from cell populations expressing a unique set of proteins defective genetic and epigenetic networks. It is shown that versican (VCAN) is upregulated by transforming growth factor-beta (TGF-ß1) in several types of cancer cell types. Our prior studies revealed that TGF-ß1 expression is elevated in cancer stem cell (CSCs) monolayer cultures and VCAN expression was also significantly increased in spheroid formed CSCs. Methods: Within the scope of these results, it was hypothesized that high TGF-ß1 expression in the monolayer might trigger the three-dimensional architectural organization by inducing VCAN expression. This study investigated the expression profiling of VCAN in primary and secondary tumor-derived cell lines and the effect of TGF-ß1 signaling in these cells. Results: The findings showed that the VCAN gene expression level in the PC3 human prostate cell line was correlated with VCAN protein expression as analyzed by western blot. There was no inducing or suppressing effect of TGF-ß1 on VCAN protein expression. Conclusion: TGF-ß1 has neither stimulatory nor inhibitory effects on VCAN expression in prostate cancer cells at the doses used in this study. In the metastatic, high VCAN protein levels were observed while no mRNA was detected which could have resulted from post-transcriptional changes such as increased protein stability and/or expression of different VCAN isoforms that may play an important role in the secondary tumor cells in prostate carcinoma.en_US
dc.identifier.doi10.4274/forbes.galenos.2022.35229
dc.identifier.endpage202en_US
dc.identifier.issn2717-9443
dc.identifier.issn2757-5241
dc.identifier.issue2en_US
dc.identifier.startpage197en_US
dc.identifier.trdizinid1120376en_US
dc.identifier.urihttps://doi.org/10.4274/forbes.galenos.2022.35229
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1120376
dc.identifier.urihttps://hdl.handle.net/11454/102734
dc.identifier.volume3en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofForbes tıp dergisi (Online)en_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240825_Gen_US
dc.titleDifferent mRNA and Protein Expression of Versican in TGF-?1- treated Prostate Cancer Cellsen_US
dc.typeArticleen_US

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