Effects of vitrification solution supplemented with platelet-rich plasma in rat ovarian tissue cryopreservation

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dc.authorscopusid57219907255
dc.authorscopusid55318368500
dc.authorscopusid57195671816
dc.authorscopusid57219905771
dc.authorscopusid57202645325
dc.authorscopusid56376463500
dc.authorscopusid55469991100
dc.contributor.authorGökhan, A.
dc.contributor.authorÇavuşoğlu, T.
dc.contributor.authorKiliç, K.D.
dc.contributor.authorŞirin, C.
dc.contributor.authorTomruk, C.
dc.contributor.authorYiğittürk, G.
dc.contributor.authorErbaş, O.
dc.date.accessioned2024-08-25T18:39:05Z
dc.date.available2024-08-25T18:39:05Z
dc.date.issued2023
dc.departmentEge Üniversitesien_US
dc.description.abstractBackground/aim: The subject of this study was to investigate the utility of platelet-rich plasma (PRP) in the cryopreservation process to reduce cryodamage and increase tissue viability. Materials and methods: Twenty-one female Wistar rats were randomly allocated to three groups. In Group 1 (G1), rats were not subjected to vitrification (n = 7). Group 2 (G2) was the vitrification group in which PRP was added to the basic vitrification solution (n = 7). Group 3 (G3) was the vitrification group in which fetal bovine serum was added to the basic vitrification solution (n = 7). Warmed tissues were evaluated with histochemical (HC) and immunohistochemical (IHC) staining, the TUNEL method, immunofluorescence (IF) staining, and biochemical analyses. Results: The percentages of IHC staining, TUNEL method positivity, and IF staining were significantly higher in G2 compared to both G1 and G3 (P < 0.05). G2 ovaries exhibited a significant increase in both malondialdehyde and catalase values in comparison to G1 (P < 0.05). In HC staining, degenerations in primary and secondary follicles and in ovarian tissue were more common in the PRP-supplemented group. The calcium used in PRP activation was suspected to have increased the degeneration and prevented the possible positive effects of PRP. Conclusion: To the best of our knowledge, PRP-supplemented vitrification solution was used for the first time in the literature in this study in whole rat ovarian tissue vitrification. If PRP is to be used as a component in vitrification solution for rat ovarian tissue, the use of lower amounts of calcium or different methods in PRP activation, or the use of nonactivated PRP, should be considered from the beginning. © TÜBİTAK.en_US
dc.description.sponsorshipEge Üniversitesien_US
dc.identifier.doi10.55730/1300-0144.5694
dc.identifier.endpage1292en_US
dc.identifier.issn1300-0144
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-85175851132en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1281en_US
dc.identifier.urihttps://doi.org/10.55730/1300-0144.5694
dc.identifier.urihttps://hdl.handle.net/11454/101220
dc.identifier.volume53en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTurkiye Kliniklerien_US
dc.relation.ispartofTurkish Journal of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240825_Gen_US
dc.subjectCalciumen_US
dc.subjectcryopreservationen_US
dc.subjectovaryen_US
dc.subjectplatelet-rich plasmaen_US
dc.subjectvitrificationen_US
dc.subjectcalciumen_US
dc.subjectcaspase 3en_US
dc.subjectcatalaseen_US
dc.subjectconnexin 43en_US
dc.subjecthydrogelen_US
dc.subjectketamineen_US
dc.subjectlactate dehydrogenaseen_US
dc.subjectmalonaldehydeen_US
dc.subjectmitochondrial permeability transition poreen_US
dc.subjectmyeloperoxidaseen_US
dc.subjectprotein bcl 2en_US
dc.subjectangiogenesisen_US
dc.subjectanimal experimenten_US
dc.subjectanimal modelen_US
dc.subjectanimal tissueen_US
dc.subjectantibody labelingen_US
dc.subjectapoptosisen_US
dc.subjectArticleen_US
dc.subjectastrocyteen_US
dc.subjectbiochemical analysisen_US
dc.subjectcell proliferationen_US
dc.subjectcell protectionen_US
dc.subjectcell survivalen_US
dc.subjectcervical spine dislocationen_US
dc.subjectcontrolled studyen_US
dc.subjectcryopreservationen_US
dc.subjectcumulus cellen_US
dc.subjectfemaleen_US
dc.subjectfertility preservationen_US
dc.subjectfetal bovine serumen_US
dc.subjectgap junctionen_US
dc.subjectgranulosa cellen_US
dc.subjecthormonal regulationen_US
dc.subjectimmunofluorescenceen_US
dc.subjectimmunohistochemistryen_US
dc.subjectlipid peroxidationen_US
dc.subjectmetaphaseen_US
dc.subjectmitochondrial membrane potentialen_US
dc.subjectnonhumanen_US
dc.subjectosmotic stressen_US
dc.subjectovariectomyen_US
dc.subjectovary follicleen_US
dc.subjectovary follicle developmenten_US
dc.subjectovary tissueen_US
dc.subjectoxidative stressen_US
dc.subjectprotein expressionen_US
dc.subjectraten_US
dc.subjectresuscitationen_US
dc.subjectspermatozoon motilityen_US
dc.subjecttestis tissueen_US
dc.subjecttheca cellen_US
dc.subjectthrombocyte rich plasmaen_US
dc.subjectTUNEL assayen_US
dc.subjectvitrificationen_US
dc.subjectWistar raten_US
dc.subjectzona pellucidaen_US
dc.titleEffects of vitrification solution supplemented with platelet-rich plasma in rat ovarian tissue cryopreservationen_US
dc.typeArticleen_US

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