Effects of vitrification solution supplemented with platelet-rich plasma in rat ovarian tissue cryopreservation
Küçük Resim Yok
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Turkiye Klinikleri
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background/aim: The subject of this study was to investigate the utility of platelet-rich plasma (PRP) in the cryopreservation process to reduce cryodamage and increase tissue viability. Materials and methods: Twenty-one female Wistar rats were randomly allocated to three groups. In Group 1 (G1), rats were not subjected to vitrification (n = 7). Group 2 (G2) was the vitrification group in which PRP was added to the basic vitrification solution (n = 7). Group 3 (G3) was the vitrification group in which fetal bovine serum was added to the basic vitrification solution (n = 7). Warmed tissues were evaluated with histochemical (HC) and immunohistochemical (IHC) staining, the TUNEL method, immunofluorescence (IF) staining, and biochemical analyses. Results: The percentages of IHC staining, TUNEL method positivity, and IF staining were significantly higher in G2 compared to both G1 and G3 (P < 0.05). G2 ovaries exhibited a significant increase in both malondialdehyde and catalase values in comparison to G1 (P < 0.05). In HC staining, degenerations in primary and secondary follicles and in ovarian tissue were more common in the PRP-supplemented group. The calcium used in PRP activation was suspected to have increased the degeneration and prevented the possible positive effects of PRP. Conclusion: To the best of our knowledge, PRP-supplemented vitrification solution was used for the first time in the literature in this study in whole rat ovarian tissue vitrification. If PRP is to be used as a component in vitrification solution for rat ovarian tissue, the use of lower amounts of calcium or different methods in PRP activation, or the use of nonactivated PRP, should be considered from the beginning. © TÜBİTAK.
Açıklama
Anahtar Kelimeler
Calcium, cryopreservation, ovary, platelet-rich plasma, vitrification, calcium, caspase 3, catalase, connexin 43, hydrogel, ketamine, lactate dehydrogenase, malonaldehyde, mitochondrial permeability transition pore, myeloperoxidase, protein bcl 2, angiogenesis, animal experiment, animal model, animal tissue, antibody labeling, apoptosis, Article, astrocyte, biochemical analysis, cell proliferation, cell protection, cell survival, cervical spine dislocation, controlled study, cryopreservation, cumulus cell, female, fertility preservation, fetal bovine serum, gap junction, granulosa cell, hormonal regulation, immunofluorescence, immunohistochemistry, lipid peroxidation, metaphase, mitochondrial membrane potential, nonhuman, osmotic stress, ovariectomy, ovary follicle, ovary follicle development, ovary tissue, oxidative stress, protein expression, rat, resuscitation, spermatozoon motility, testis tissue, theca cell, thrombocyte rich plasma, TUNEL assay, vitrification, Wistar rat, zona pellucida
Kaynak
Turkish Journal of Medical Sciences
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
53
Sayı
5
