An alginate-poly(acrylamide) hydrogel with TGF-beta 3 loaded nanoparticles for cartilage repair: Biodegradability, biocompatibility and protein adsorption

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dc.contributor.authorSaygili, Ecem
dc.contributor.authorKaya, Elif
dc.contributor.authorIlhan-Ayisigi, Esra
dc.contributor.authorSaglam-Metiner, Pelin
dc.contributor.authorAlarcin, Emine
dc.contributor.authorKazan, Aslihan
dc.contributor.authorAkakin, Dilek
dc.date.accessioned2021-05-03T20:27:40Z
dc.date.available2021-05-03T20:27:40Z
dc.date.issued2021
dc.departmentEge Üniversitesien_US
dc.description.abstractCurrent implantable materials are limited in terms of function as native tissue, and there is still no effective clinical treatment to restore articular impairments. Hereby, a functionalized polyacrylamide (PAAm)-alginate (Alg) Double Network (DN) hydrogel acting as an articular-like tissue is developed. These hydrogels sustain their mechanical stability under different temperature (+4 degrees C, 25 degrees C, 40 degrees C) and humidity conditions (60% and 75%) over 3 months. As for the functionalization, transforming growth factor beta-3 (TGF-beta 3) encapsulated (NPTGF-beta 3) and empty poly(lactide-co-glycolide) (PLGA) nanopartides (PLGA NPs) are synthesized by using microfluidic plat-form, wherein the mean particle sizes are determined as 81.94 +/- 92 nm and 126 +/- 4.52 nm with very low poly-dispersity indexes (PDI) of 0.194 and 0.137, respectively. Functionaliza lion process of PAAm-Alg hydrogels with ester-end PLGA NPs is confirmed by MR analysis, and higher viscoelastidty is obtained for functionalized hydrogels. Moreover, cartilage regeneration capability of these hydrogels is evaluated with in vitro and in vivo experiments. Compared with the PAAm-Alg hydrogels, functionalized formulations exhibit a better cell viability. Histological staining, and score distribution confirmed that proposed hydrogels significantly enhance regeneration of cartilage in rats due to stable hydrogel matrix and controlled release of TGF-beta 3. These findings demonstrated that PAAm-Alg hydrogels showed potential for cartilage repair and clinical application. (C) 2021 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [117M843]; National Research Founda-tion of KoreaNational Research Foundation of Korea [2017K2A9A1A06037807]en_US
dc.description.sponsorshipThis work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) under grant number 117M843. Y.-W.KimandJ.-Y.Sun were supported under the framework of international cooperation program managed by the National Research Founda-tion of Korea (2017K2A9A1A06037807, FY2017).en_US
dc.identifier.doi10.1016/j.ijbiomac.2021.01.069en_US
dc.identifier.endpage393en_US
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.pmid33476613en_US
dc.identifier.scopus2-s2.0-85099620615en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage381en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2021.01.069
dc.identifier.urihttps://hdl.handle.net/11454/69612
dc.identifier.volume172en_US
dc.identifier.wosWOS:000619184100039en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofInternational Journal of Biological Macromoleculesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlginateen_US
dc.subjectArticular cartilageen_US
dc.subjectDouble network hydrogelsen_US
dc.subjectNanoparticlesen_US
dc.subjectGrowth factoren_US
dc.titleAn alginate-poly(acrylamide) hydrogel with TGF-beta 3 loaded nanoparticles for cartilage repair: Biodegradability, biocompatibility and protein adsorptionen_US
dc.typeArticleen_US

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