G protein-coupled estrogen receptor1 (GPER1) may mediate Rho-kinase (ROCK-2) up-regulation in coronary endothelial cells

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dc.contributor.authorKurt A.H.
dc.contributor.authorTiftik R.N.
dc.contributor.authorUn I.
dc.contributor.authorUlker S.
dc.contributor.authorBuyukafsar K.
dc.date.accessioned2019-10-26T21:36:20Z
dc.date.available2019-10-26T21:36:20Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractobjective. Efect of estrogenic compounds and 17ß-estradiol (E2), which induces endothelial cell motility, was investigated on ROCK-2 expression in rat coronary vascular endothelial cells (CVEC). Methods. Te CVEC were isolated from the heart of Wistar rats by collagenase (0.04%) and incubated with E2 (1-100 nM), estrogen receptor ? (ER?) agonist: propyl pyrazole triol (PPT, 10 nM); ERß agonists: (2,3-bis(4-hydroxyphenyl)-propionitrile, DPN, 10 nM) and E2-conjugate with bovine serum albumin (E2-BSA, 1 nM); and GPER1 agonist: G1 (100 nM). Furthermore, the efect of combination of E2 with estrogen receptors (ERs) antagonist and GPER1 agonist, ICI-182780 (10 µM), physiological estrogen antagonists: progesterone (P4, 10-100 nM) and testosterone (T, 10-100 nM); transcription inhibitor: actinomycin-D (1 µg/ml); GPER1 antagonist: G-15 (100 nM), superoxide dismutase, (SOD, 500 U/ml); Gi/o protein inhibitor: pertussis toxin (PTX, 100 µg/ml); and epidermal growth factor receptor (EGFR) blocker: AG-1478 (10 µM) was tested. After 24h incubation, ROCK-2 and GPER1 protein expressions were detected in the CVEC by Western-blotting. results. E2, ICI-182780, and G1 but not E2-BSA signifcantly up-regulated ROCK-2 expression, which was suppressed by actinomycin-D, PTX, AG-1478, and G-15. However, PPT and DPN had no efects on the ROCK-2 expression. ICI-182780, P4, T or SOD did not antagonize the E2 action. GPER1 expression was demonstrated in the CVEC. Conclusions. Estrogens could up-regulate ROCK-2 in the rat CVEC through GPER1 and EGFR transactivation.en_US
dc.identifier.doi10.4149/endo_2013_02_75en_US
dc.identifier.endpage84en_US
dc.identifier.issn1210-0668
dc.identifier.issue2en_US
dc.identifier.pmid23641788en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage75en_US
dc.identifier.urihttps://doi.org/10.4149/endo_2013_02_75
dc.identifier.urihttps://hdl.handle.net/11454/17998
dc.identifier.volume47en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofEndocrine Regulationsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject17ß-estradiolen_US
dc.subjectEndotheliumen_US
dc.subjectGPER1en_US
dc.subjectICI-182780en_US
dc.subjectRho-kinaseen_US
dc.titleG protein-coupled estrogen receptor1 (GPER1) may mediate Rho-kinase (ROCK-2) up-regulation in coronary endothelial cellsen_US
dc.typeArticleen_US

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