Design and evaluation of an intravesical delivery system for superficial bladder cancer: preparation of gemcitabine HCl-loaded chitosan-thioglycolic acid nanoparticles and comparison of chitosan/poloxamer gels as carriers

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dc.contributor.authorSenyigit, Zeynep Ay
dc.contributor.authorKaravana, Sinem Yaprak
dc.contributor.authorIlem-Ozdemir, Derya
dc.contributor.authorCaliskan, Cagri
dc.contributor.authorWaldner, Claudia
dc.contributor.authorSen, Sait
dc.contributor.authorBernkop-Schnurch, Andreas
dc.contributor.authorBaloglu, Esra
dc.date.accessioned2019-10-27T22:29:16Z
dc.date.available2019-10-27T22:29:16Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractThis study aimed to develop an intravesical delivery system of gemcitabine HCl for superficial bladder cancer in order to provide a controlled release profile, to prolong the residence time, and to avoid drug elimination via urination. For this aim, bioadhesive nanoparticles were prepared with thiolated chitosan (chitosan-thioglycolic acid conjugate) and were dispersed in bioadhesive chitosan gel or in an in situ gelling poloxamer formulation in order to improve intravesical residence time. In addition, nanoparticle-loaded gels were diluted with artificial urine to mimic in vivo conditions in the bladder and were characterized regarding changes in gel structure. The obtained results showed that chitosan-thioglycolic acid nanoparticles with a mean diameter of 174.5 +/- 3.762 nm and zeta potential of 32.100 +/- 0.575 mV were successfully developed via ionotropic gelation and that the encapsulation efficiency of gemcitabine HCl was nearly 20%. In vitro/ex vivo characterization studies demonstrated that both nanoparticles and nanoparticle-loaded chitosan and poloxamer gels might be alternative carriers for intravesical administration of gemcitabine HCl, prolonging its residence time in the bladder and hence improving treatment efficacy. However, when the gel formulations were diluted with artificial urine, poloxamer gels lost their in situ gelling properties at body temperature, which is in conflict with the aimed formulation property. Therefore, 2% chitosan gel formulation was found to be a more promising carrier system for intravesical administration of nanoparticles.en_US
dc.description.sponsorshipScientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TUBITAK-112/S/293]; TR Prime Ministry State Planning Organization FoundationTurkiye Cumhuriyeti Kalkinma Bakanligi [09/DPT/001]en_US
dc.description.sponsorshipThis study was supported by The Scientific and Technological Research Council of Turkey (grant number TUBITAK-112/S/293). The authors would also like to thank to the TR Prime Ministry State Planning Organization Foundation (Project Number 09/DPT/001).en_US
dc.identifier.doi10.2147/IJN.S93750en_US
dc.identifier.issn1178-2013
dc.identifier.pmid26508855en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.2147/IJN.S93750
dc.identifier.urihttps://hdl.handle.net/11454/51074
dc.identifier.volume10en_US
dc.identifier.wosWOS:000363190800001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherDove Medical Press Ltden_US
dc.relation.ispartofInternational Journal of Nanomedicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectchitosan-TGAen_US
dc.subjectnanoparticleen_US
dc.subjectgemcitabine HClen_US
dc.subjectintravesical administrationen_US
dc.subjectchitosanen_US
dc.subjectpoloxameren_US
dc.subjectsuperficial bladder canceren_US
dc.titleDesign and evaluation of an intravesical delivery system for superficial bladder cancer: preparation of gemcitabine HCl-loaded chitosan-thioglycolic acid nanoparticles and comparison of chitosan/poloxamer gels as carriersen_US
dc.typeArticleen_US

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