Matrix metalloproteinases, tissue inhibitor of matrix metalloproteinase-1, and laminin-5 gamma 2 chain immunolocalization in gingival tissue of endotoxin-induced periodontitis in rats: Effects of low-dose doxycycline and alendronate

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dc.contributor.authorBuduneli, Eralp
dc.contributor.authorVardar-Sengul, Saynur
dc.contributor.authorBuduneli, Nurcan
dc.contributor.authorAtilla, Gul
dc.contributor.authorWahlgren, Jaana
dc.contributor.authorSorsa, Timo
dc.date.accessioned2019-10-27T19:38:23Z
dc.date.available2019-10-27T19:38:23Z
dc.date.issued2007
dc.departmentEge Üniversitesien_US
dc.description.abstractBackground: Matrix metalloproteinases (MMPs) play important roles in tissue destruction mechanisms of periodontitis. MMP-8 and -13 are the major collagenases that act in extracellular matrix degradation in periodontal tissues. MMP-14 is a membrane-type MMP, and laminin (Ln)-5 is a basal membrane component. The aim of the present study was to evaluate the effects of doxycycline and alendronate on gingival tissue expression of MMP-8, -13, and -14; tissue inhibitors of MMP (TIMP)-1; and Ln-5 gamma 2 chain in experimental periodontitis induced by Escherichia coli endotoxin (LPS) in rats. Methods: Experimental periodontitis was induced by repeated injection of LPS. Forty-four adult male Sprague-Dawley rats were divided into five study groups: saline control, LPS, LPS + doxycycline, LPS + alendronate, and LPS + doxycycline + alendronate. Doxycycline and alendronate were given as a single agent or as combination therapy during the 7 days of the experimental study period. On day 7, the rats were sacrificed, and the gingival tissues were analyzed immunohistochemically for expression of MMP-8, -13, and -14, Ln-5 gamma 2 chain, and TIMP-1. Alveolar bone loss was evaluated morphometrically under a stereomicroscope. Data were tested statistically by Kruskal-Wallis and Mann-Whitney tests and Spearman correlation analysis. Results: Alveolar bone loss was significantly higher in the LPS, doxycycline, alendronate, and combination groups than in the saline control group (all P < 0.01). MMP-8 expression was significantly higher in the LPS group than in the saline control group (P = 0.001). Individual administration of doxycycline or alendronate significantly decreased the expression of MMP-8 compared to LPS (P = 0.01). Combined drug administration reduced MMP-14 significantly compared to doxycycline (P = 0.004). No significant differences in Ln-5 gamma 2 chain expression were found between the study groups (P > 0.05). MMP-14 significantly correlated with the Ln-5 gamma 2 chain in the LPS + alendronate group (P = 0.04) and with the amount of alveolar bone loss in the LPS + doxycycline + alendronate group (P = 0.03). Conclusions: Our findings suggest that alendronate and/or doxycycline may inhibit MMP-8 expression significantly; particularly, their combined administration may provide beneficial effects in periodontal treatment. Moreover, individual administration of alendronate and doxycycline results in significant increases in TIMP-I expression in gingiva. However, these effects of combined low-dose doxycycline and alendronate on MMPs and TIMP should be verified by clinical human trials before these agents are used in dental practice.en_US
dc.identifier.doi10.1902/jop.2007.050451en_US
dc.identifier.endpage134en_US
dc.identifier.issn0022-3492
dc.identifier.issue1en_US
dc.identifier.pmid29539192en_US
dc.identifier.startpage127en_US
dc.identifier.urihttps://doi.org/10.1902/jop.2007.050451
dc.identifier.urihttps://hdl.handle.net/11454/40079
dc.identifier.volume78en_US
dc.identifier.wosWOS:000243572900018en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmer Acad Periodontologyen_US
dc.relation.ispartofJournal of Periodontologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectalendronateen_US
dc.subjectbisphosphonatesen_US
dc.subjectdoxycyclineen_US
dc.subjectlaminin 5en_US
dc.subjectMMPsen_US
dc.subjectTIMP-1en_US
dc.titleMatrix metalloproteinases, tissue inhibitor of matrix metalloproteinase-1, and laminin-5 gamma 2 chain immunolocalization in gingival tissue of endotoxin-induced periodontitis in rats: Effects of low-dose doxycycline and alendronateen_US
dc.typeArticleen_US

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