Development and validation of an HPLC method for the determination of hyaluronic acid active substance in pharmaceutical formulations
Küçük Resim Yok
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Marmara Univ
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
It is an analytical requirement to identify and determine high molecular weight APIs in pharmaceutical forms. For this reason, it is aimed to develop and validate an analytical method for the determination of hyaluronic acid in pharmaceutical form in this study. 0.1 M Na2SO4 was prepared as mobile phase. The separation of compound was performed with a OHpak Shodex SB-806M HQ (13 mu m, 8,0 x 300mm, Japan) analytical column. Guard column which is OHpak SB-G, (8 mu m,100 A, 6.0 x 50 mm, Japan) was integrated to the system before the analytical column. UV detection at 198 nm was used to monitor the eluent and flow rate of the mobile phase was set to 1 mL/min. The method has been validated in terms of system suitability, linearity, limits of detection (LOD) and quantity (LOQ), precision, accuracy, specificity, selectivity, and stability. The obtained findings showed that the analytical method has linearity higher than 0.99, accuracy, precision, selectivity and stability. The method was found to be precise, accurate and specific during the study.
Açıklama
Anahtar Kelimeler
Hyaluronic acid, HPLC-UV, microemulsion, analytical method, validation, Microemulsion Formulations, Synovial-Fluid, Adhesions, Hydrogels, Delivery
Kaynak
Journal Of Research In Pharmacy
WoS Q Değeri
N/A
Scopus Q Değeri
Q3
Cilt
26
Sayı
1
