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Öğe Beyond Natural Antioxidants in Cancer Therapy: Novel Synthetic Approaches in Harnessing Oxidative Stress(Springer Singapore, 2022) Moulahoum, H.; Ghorbanizamani, F.; Timur, S.; Zihnioglu, F.Oxidative stress is a physiological phenomenon that provides favorable outcomes such as in inflammation processes. It has also a harmful side that when the level of reactive oxygen species produced is higher than the capacity of the cell to neutralize them, this imbalance can develop into a precursor of some pathological conditions or enhance already present conditions. On the other hand, scientists have been exploring the oxidative stress system for decades and learned how to harness this system in therapy. The most popular approach is the use of natural and dietary antioxidant to target oxidative stress and induce therapy. However, other than the natural sources, scientists seek many synthetic and novel molecules such as peptide hybrids, polymers, and nanoparticles, which contain an inherent antioxidant activity in their structures. In this chapter, we tried to focus on the novel experimental therapeutic approaches used in pathology and chemotherapy. We hope the colleagues will be able to garner an interest to such approaches and further harness the oxidative stress in their research using these new tools to enhance biomedicine and therapeutics. © Springer Nature Singapore Pte Ltd. 2022.Öğe Characterization of recombinant Prolyl oligopeptidase from Myxococcus Xanthus and potential use in gluten hydrolysis(Wiley-Blackwell, 2016) Kocaazorbaz, E. K.; Zihnioglu, F.Öğe Dipeptidyl peptidase IV inhibitory activity of Arctium tomentosum L.(Wiley-Blackwell, 2016) Zeytunluoglu, A.; Zihnioglu, F.Öğe How should diagnostic kits development adapt quickly in COVID 19-like pandemic models? Pros and cons of sensory platforms used in COVID-19 sensing(Elsevier B.V., 2021) Moulahoum, H.; Ghorbanizamani, F.; Zihnioglu, F.; Turhan, K.; Timur, S.As COVID-19 has reached pandemic status and the number of cases continues to grow, widespread availability of diagnostic testing is critical in helping identify and control the emergence of this rapidly spreading and serious illness. However, a lacking in making a quick reaction to the threat and starting early development of diagnostic sensing tools has had an important impact globally. In this regard, here we will review critically the current developed diagnostic tools in response to the COVID-19 pandemic and compare the different types through the discussion of their pros and cons such as nucleic acid detection tests (including PCR and CRISPR), antibody and protein-based diagnosis tests. In addition, potential technologies that are under development such as on-site diagnosis platforms, lateral flow, and portable PCR units are discussed. Data collection and epidemiological analysis could also be an interesting factor to incorporate with the emerging technologies especially with the wide access to smartphones. Lastly, a SWOT analysis and perspectives on how the development of novel sensory platforms should be treated by the different decision-makers are analyzed. © 2020 Elsevier B.V.Öğe Nanoconjugated materials as sensors in point-of-care diagnostic tools: Detection of small molecules and viruses(Elsevier B.V., 2023) Moulahoum, H.; Ghorbanizamani, F.; Guler, Celik, E.; Zihnioglu, F.; Timur, S.Diagnostic point of care (POC) tools have seen important advances through the many materials introduced to enhance and validate their wide range applications. One of the most used POC tools are paper-based colorimetric formats. These POC are generally based on the use of antibody-antigen pairs interaction for the detection. However, small molecules can be a challenge for these formats and drastically reduce the sensitivity of POC. Therefore, novel conjugated materials using nanoparticles, polymers, and other composites have been developed which helped to tackle the sensitivity issues and, by using these materials, the portable sensors became more trustworthy for the detection of small molecules. These materials can be sculpted into various nanostructures and networks such as nanovesicles and nanogels with high biocompatibility and tunability. These are regarded as promising tools in the current and future lab-on-chip devices due to their accessibility and ease to manufacturing. In addition, the application of portable biosensing devices is of great importance in large-scale screenings of viruses including the coronavirus SARS-CoV-2 (responsible for the COVID-19 pandemic) or road control (i.e., substance of abuse). These approaches were made more accessible using smartphone-assisted analyses allowing for the decentralization of diagnosis. In this chapter, we present the latest findings in the development of polymeric-based materials and biosensors aimed for the detection of viruses and small molecules of drug abuse through simplified approaches including colorimetric paper-based assays and electrochemical sensors. The use of nano-scaled bioconjugated materials became an integral component in sensing applications due to their various structural advantages in producing highly sensitive tools that rival bench-top instruments. New developments in material design opened the door for decentralized dispensation of medicines and public protection that allows effective onsite and point-of-care diagnostics. © 2023 Elsevier B.V.Öğe Participating in scientific meetings as part of PhD training: 6 years of educational and scientific data(Wiley, 2022) Kocdor, H.; Harmanci, D.; Pabuccuoglu, A.; Zihnioglu, F.; Yener, N.; Sagin, F.[No Abstract Available]Öğe Passive targeting therapy for brain delivery nanocarriers using natural dipeptide antioxidant L-carnosine-loaded niosomes(Wiley, 2019) Moulahoum, H.; Zamani, F. Ghorbani; Timur, S.; Zihnioglu, F.[No abstract available]Öğe Purification of Leptinotarsa decemlineata (Say) Gut Specific Cysteine Protease Inhibitor(s) From Rapeseed(Tarbiat Modares Univ, 2017) Ashouri, Sh.; Zihnioglu, F.; Pourabad, R. Farshbaf; Kocadag, E.The aim of the present work was to purify cysteine protease inhibitors from rapeseed (Brassica napus L.), with potential activity on digestive protease of Colorado Potato Beetle (CPB), Leptinotarsa decemlineata (Say). Ammonium sulfate precipitated proteinaceous fractions; 30, 50, 70, and 100% showed 39.07, 57.03, 51.47, and 22.44% inhibition on the fourth instar larval gut general protease activity, respectively. Fraction 50% showed the highest inhibitory effect on digestive general protease activity of all developmental stages. Gel assays approved the inhibition of the enzyme activity. Fraction 50% was purified by using various chromatography techniques; ion-exchange using DEAE, gel filtration and affinity using SiO2-CPB larval gut homogenate. Three peaks of protein were eluted from ion exchange chromatography using NaCl step gradient, also from gel filtration chromatography. When Z-Ala-Arg-Arg-4mBNA was used as cysteine protease substrate, the purification fold of second fraction of ion exchange chromatography was obtained 24.80, also the yield was 59.09%, the third fraction of gel permeation resulted in a 25.60 fold purification with 28.53% of recovery, and the fraction of affinity chromatography obtained a 22.72 fold purification and yielded 36.35%. In the SDS-PAGE, apparent molecular mass of purified proteins were 34 and 32 kDa by ion-exchange and 24 and 22 kDa by affinity. However, gel filtration was not an appropriate method in this study, because the purified protein band(s) were not observed on the gel. Consequently, these chromatography methods were appropriate methods to purification of inhibitor cystatins, specially affinity which was prepared by using CPB gut enzyme as ligand and obtained specific inhibitor proteins of CPB gut protease activity.Öğe Role of phytochemicals and secondary metabolites from Mentha spicata in acetylcholine esterase inhibition for effective pest control of Tetranychus urticae Koch(Taylor and Francis Ltd., 2023) Mammadova, T.; Guliyeva, L.; Moulahoum, H.; Tok, K.; Küçükçobanoğlu, Y.; Yildiz, Aktas, L.; Zihnioglu, F.The two-spotted spider mite (Tetranychus urticae Koch) poses a significant threat to global food production. However, the environmental risks associated with synthetic pesticides have necessitated the exploration of eco-friendly alternatives. Plant-based biopesticides utilizing secondary metabolites have emerged as promising strategies for pest management. This study aimed to extract and characterize Mentha spicata L. through biochemical analyses, GC-MS, and LC-QTOF-MS. The extract’s acaricidal, oviposition deterrence, and repellent properties were evaluated against Tetranychus urticae, revealing a pronounced time- and concentration-dependent effect over 96 hours, with an LC50 of 3 µg/mL. Furthermore, the impact of the extract on plant growth was assessed using tomato plants, demonstrating an enhancement in growth rate based on photosynthesis, weight, and oxidative stress markers analyses. Polyphenolics and terpenoids in M. spicata were identified as contributors to its biological activities, including antioxidant and free-radical scavenging properties. Molecular docking experiments targeting acetylcholine esterase (AChE) were conducted on the identified phytocompounds, indicating a high affinity for ?-3-Carene and Isoferuloyl C1 glucuronide. The pesticidal effects of these compounds were attributed to their competitive and synergistic inhibition of AChE. These findings highlight the potential of plant extracts, specifically M. spicata, as effective biopesticides in future pest control strategies. The utilization of secondary metabolites from plant sources offers a sustainable and environmentally friendly approach to combatting T. urticae infestations. © 2023 Informa UK Limited, trading as Taylor & Francis Group.Öğe Scintigraphic imaging with a peptide glucuronide in rabbits: Tc-99m-exorphin C glucuronide(Pergamon-Elsevier Science Ltd, 2007) Ertay, T.; Unak, P.; Biber, F. Z.; Tasci, C.; Zihnioglu, F.; Durak, H.A peptide glucuronide (Exorphin C glucuronide) was labeled with Tc-99m using glucoheptonate (GH) as a bifunctional chelating agent. Scintigraphic imaging was performed in male Albino rabbits. Exorphin C glucuronide showed rapid and efficient labeling with Tc-99m using glucoheptonate as a bifunctional chelate. Results demonstrated that Tc-99m-GEG may be a useful new type of glucuronide derivative of peptides for diagnosis of some cancer diseases. (c) 2006 Elsevier Ltd. All rights reserved.Öğe Tc-99m-exorphin-glucuronide in tumor diagnosis: Preparation and biodistribution studies in rats(Springer, 2006) Ertay, T.; Unak, P.; Ozdogan, O.; Biber, F. Z.; Zihnioglu, F.; Medine, E. I.; Durak, H.A glucuronide derivative of exorphin C called exorphin C glucuronide was obtained by enzymatic synthesis and labeled with Tc-99m using glucoheptonate (GH) as bifunctional chelating agent. Labeling efficiency was 99.7 +/- 0.03%. Biodistribution studies were performed on normal and mammary tumor bearing albino Wistar rats. In addition, scintigraphic imaging with Tc-99m-GH-exorphin C glucuronide (Tc-99m-GEG) was performed. The radiopeptide-glucuronide is rapidly cleared via the urinary system showing lower liver uptake. Results demonstrated that Tc-99m-GEG can be used as a vehicle to target tumors.Öğe Total proteoglycan and chondroitin-4-sulphate levels in specific periodontal diseases.(Int Amer Assoc Dental Researchi A D R/A A D R, 2003) Vardar, S.; Baylas, H.; Zihnioglu, F.; Emingil, G.; Buduneli, N.; Atilla, G.Öğe Total proteoglycan and chondroitin-4-sulphate levels in specific periodontal diseases.(Int Amer Assoc Dental Researchi A D R/A A D R, 2003) Vardar, S.; Baylas, H.; Zihnioglu, F.; Emingil, G.; Buduneli, N.; Atilla, G.
